微波消融联合5-氟尿嘧啶瘤内注射对小鼠结肠癌移植瘤的治疗

Therapeutic effect of microwave ablation combined with injection of 5-? uorouracil in mice bearing CT26 colon carcinoma xenografts

目的:研究微波消融(MA)联合5-氟尿嘧啶瘤内注射对小鼠移植性结肠癌的治疗作用.方法:Balb/c小鼠皮下接种结肠癌CT26细胞建立肿瘤模型,肿瘤分别给予瘤周注射PBS、MA、瘤周注射5-氟尿嘧啶和MA+瘤周注射5-氟尿嘧啶4种处理.测量各组肿瘤大小;观察肿瘤复发情况、动物生存期及生活状态;ELISA法检测各组小鼠外周血TNF-α、IL-2、IL-12和IFN-γ的含量.结果:60d生存期观察发现微波消融组47d±1.7d、5-氟尿嘧啶组36d±2.1d和微波消融联合5-氟尿嘧啶组56d±2.5d小鼠的生存期显著高于PBS组33d±1.4d;微波消融联合5-氟尿嘧啶组肿瘤体积[(0.11±0.03)cm×cm×cm]较其他3组显著增大(P<0.05);联合治疗组小鼠外周血中TNF-α浓度(ng/L),IL-2浓度(ng/L),IL-12浓度(ng/L),IFN-γ浓度(ng/L)显著高于其他3组(180.3±35.9vs62.8±12.7,96.3±25.1,136.9±49.4;72.5±6.8vs20.6±7.3,27.9±10.5,46.4±9.3;302.3±69.6vs123.4±13.1,153.8±35.4,278.9±57.0;68.4±8.2vs29.9±4.3,36.8±4.6,59.1±9.0,均P<0.01).结论:MA联合5-氟尿嘧啶瘤内注射可以减少结肠癌移植瘤局部复发,提高治疗效果.

AIM： To evaluate the therapeutic effect of micro- wave ablation （MA） combined with injection of 5-fluorouracil （5-FU） in mice bearing CT26 colon carcinoma xenografts. METHODS： Balb/c mice were subcutaneously inoculated with CT26 cells to establish a xenograft mouse model of colon carcinoma. Model mice were divided into four groups： PBS group, MA group, 5-FU group, and MA plus 5-FU group. After treatment, the changes in tumor volume were measured; tumor recurrence, mouse survival and living status were observed; and peripheral serum levels of TNF-α, IL-2, IL-12 and IFN-γ were determined by ELISA.RESULTS： The life span of mice treated by MA, 5-FU injection, MA plus 5-FU was significantly longer than that of mice treated with PBS （47 d ± 1.7 d, 36 d ± 2.1 d, 56 d ± 2.5 d vs 33 d ± 1.4 d, all P 〈 0.05）. Tumor size significantly decreased in the MA plus 5-FU group compared to the other three groups （all P 〈 0.05）. Peripheral serum levels of TNF-α, IL-2, IL-12, and IFN-γ in the combination therapy group were significantly higher than those in the other three groups （180.3 ± 35.9 vs 62.8 ± 12.7, 96.3 ± 25.1, 136.9 ± 49.4; 72.5 ± 6.8 vs 20.6 ± 7.3, 27.9 ± 10.5, 46.4 ± 9.3; 302.3 ± 69.6 vs 123.4 ± 13.1, 153.8 ± 35.4, 278.9 ± 57.0; 68.4 ± 8.2 vs 29.9 ± 4.3, 36.8 ± 4.6, 59.1 ± 9.0; all P 〈 0.01）.CONCLUSION： MA combined with 5-FU injection can improve anti-tumor effect by strengthening immunological function and reducing local recurrences as compared to that of either treatment alone.