小麦冠腐病菌(Fusarium pseudograminearum)FPCS3096菌株产孢条件的研究

Studies on Conditions for Sporulation of Pathogen Fusarium pseudograminearum

由真菌Fusarium pseudograminearum引起的小麦冠腐病是澳大利亚小麦生产上最严重的病害。为进行病害抗性鉴定和筛选抗病种质资源,需制备大量的病菌分生孢子。本试验以强致病性菌株FPCS3096为材料,开展了该菌株产孢条件的研究,结果表明：在6种培养基中,以1/2 PDA产孢量最大;在营养生长阶段,在菌丝长满平板后（28℃下需培养6~7 d）,延长培养时间（即继续培养）,大大促进了后期的产孢,培养12 d的产孢量是培养6 d的4倍,而且在这一阶段,暗培养促进了产孢,连续暗培养的产孢数是连续光培养产孢数的2倍多。试验同时发现,在一定的范围内,培养皿内培养基的重量或平板培养基的厚度对产孢有很大的影响,量越多,产孢量越大,当培养基的重量比为2倍多时,产孢的数量比为7~10倍。与本实验室以前采用的在PDA培养基上培养、然后光照产孢的技术相比,现有的技术将产孢数量提高了20~50倍。

Fusarium pseudograminearum（FPCS3096）,the causal agent of wheat crown rot,is an economically important disease in Australia.The studies on conditions for sporulation of the pathogen were conducted in order to increase amount of sporlution for the purpose of artificial inoculation.The results showed that the amount of sporulation of FPCS3096 on 1/2PDA medium was the biggest of 6 kinds of media;that prolonging incubation time after the mycelium spreads all the plate（it took 6-7days at 28 ℃） during the mycelium growth stage would greatly enhance sporulation during the sporulation stage,and the amount of sporulation at a 12-days long incubation period was 4 times as high as that at a 6-days period;that also dark incubation during the mycelium growth stage enhanced the sporulation as compared with light incubation,and the amount of sporulation at a photoperiod of 24 h darkness incubation was more than 2 times as high as that at a photoperiod of 24 h light incubation;that the weight of medium poured into plates influenced the sporlution,and the more weight,and the greater amount of sporlution.The current technique increases amount of sporlution