茉莉酸甲酯对水稻幼苗抗细菌性条斑病的诱导效应

Effects of Methyl Jasmonate on Induced Resistance of Rice Seedlings against Bacterial Leaf Stripe

以4叶1心期水稻幼苗为试验材料,用茉莉酸甲酯（methyl jasmonate,MeJA）处理后剪叶接种浓度为5×108cfu/mL的水稻细菌性条斑病菌液,以探索MeJA对水稻细菌性条斑病抗性的诱导效应。结果表明：MeJA诱导抗病性强弱与其浓度和处理时间有关。0.01~5.0 mmol/L MeJA处理水稻幼苗5 d后均能显著抑制细条病的发生,其中0.1,0.5,1.0 mmol/L MeJA诱导效果达43.0%以上,高于0.01和5.0 mmol/L,后者分别为19.6%和21.5%;0.5 mmol/L MeJA处理水稻幼苗,1、3、5、7和10 d后接种,病情指数均明显低于对照,诱导效果依次为25.6%、31.3%、51.3%、54.6%和47.8%。

Experiments were carried out to study the inducing effects of MeJA on resistance against rice bacterial leaf stripe by spraying the 4-leaf rice seedlings with MeJA and then inoculating with a population density of 5×108 cfu/ml of Xanthomonas oryzae pv.oryzicola.The results showed that the induced resistance was associated with the concentration of MeJA and the interval between MeJA spraying and inoculation with Xanthomonas oryzae pv.oryzicola.The efficient concentration of MeJA to induce resistance against rice bacterial leaf stripe was in a range of 0.01~5.0 mmol/L,when inoculation was conducted 5 days after MeJA treatment.The induced effects of 0.1,0.5 and 1.0 mmol/L MeJA were more than 43.0%,better than those of 0.01 and 5 mmol/L MeJA,which were 19.6% and 21.5% respectively.The disease indexes of MeJA treated seedlings were obviously lower than that of CK when the inoculation was conducted 1,3,5,7 and 10 d after 0.5 mmol/L MeJA treatment,with the induced effects of 25.6%,31.3%,51.3%,54.6%,and 47.8% respectively.