杨树次生代谢物质与青杨天牛酶活性的关系

Relationship between Secondary metabolites of Poplar and Enzymatic Activity of Saperda populnea

选取黑林1号杨树内10种次生代谢物质,制成半人工饲料对青杨天牛幼虫进行饲喂,调查幼虫死亡率,同时测定幼虫体内3种解毒酶(谷胱甘肽-S-转移酶、羧酸酯酶和α-乙酸萘酯酶)和2种保护酶(SOD,CAT)的活力指标。结果表明:饲喂2.00,20.00mg·g-1的丁香酸,16.00mg·g-1的苯酚,8.00mg·g-1的肉桂酸,50.00mg·g-1的对羟基苯甲酸和4.00mg·g-1的绿原酸对青杨天牛幼虫的死亡有显著影响。经次生代谢物质饲喂后,幼虫体内保护酶SOD活性显著升高,而不同次生代谢物质对虫体CAT活性无显著影响,谷胱甘肽-S-转移酶和羧酸酯酶活性较对照提高不显著,单宁饲喂后,幼虫体内α-乙酸萘酯酶的活性显著提高。

In order to study the relationship between secondary metabolism substances in poplar and resistance of Saperda populnea L.to the substances,the insects were fed with 10 kinds of secondary metabolism substances,selected based on previous researches.The larvae mortality and activities of enzymes,including SOD,CAT,glutathione s-transferase,carboxylesterase and α-napthyl acetate,in the larvae were investigated.Results showed that 2.00 mg·g-1 and 20.00 mg·g-1 syringic acid,16.00 mg·g-1 phenol,8.00 mg·g-1 trans-cinnamic acid,50.00 mg·g-1 4-hydroxybenzoic acid and 4.00 mg·g-1 chlorogenic acid increased mortality of the larvae.Meanwhile the activity of SOD in larvae increased significantly,after being fed with secondary metabolism materials.The activity of SOD of larvae increased significantly after fed with secondary metabolism materials,and the activity of α-napthyl acetate was increased by tannin.However,secondary metabolism materials had no obvious effect on CAT and glutathione S-tramsferases of S.populnea.