杭州地区性病门诊患者人乳头状瘤病毒临床流行病学研究

Epidemiological investigation of human papillomavirus in venereal disease clinics,Hangzhou,Zhejiang

目的了解杭州地区性病门诊患者人乳头状瘤病毒(HPV)临床流行病学情况,为HPV感染的防治提供依据。方法选择300例性病门诊患者,其中150例尖锐湿疣(CA)患者和150例无CA的患者。CA患者取疣体组织,无CA患者男性取龟头和冠状沟表面细胞组织,女性取宫颈管细胞组织,提取DNA后进行HPV基因芯片杂交分型。结果 300例患者中单一型HPV感染150例,双重、三重、四重和五重HPV感染分别为24例、10例、6例和3例。CA患者和无CA患者均以低危型HPV6和11最常见,高危型则以HPV16、33、52、58和68较为常见。150例无CA的患者中也有55例HPV检测阳性,阳性率高达36.7%。女性HPV多重感染性率16.42%高于男性的3.6%(P<0.01)。结论杭州地区性病门诊患者以低危型HPV6和11感染为主,无CA的性病门诊患者也具有较高的HPV感染率。

Objective To investigate the infection status of human papillomavirus（HPV） in the patients of venereal disease clinics and provide epidemiological evidence for HPV prevention.Methods A group of 300 cases of venereal disease clinic patients were enrolled from Hangzhou,Zhejiang,including 150 cases each of condyloma acuminate（CA） and non-CA patients.Specimens collected included wart tissues from CA patients,the surface cells of balanus as well as atrioventricular furrow cells and cervical canals cells from non-CA male and female patients,respectively.DNA was extracted and the method of gene chip was used for HPV strain typing.Results Among 300 cases of venereal disease clinic patients,50%（150）,8%（24）,3.33%（10）,2%（6） and 1%（3） of patients were infected with one,two,three,four and five type（s） HPV respectively.HPV 6 and HPV 11 were the most common detected HPV types in patients with or without CA.The most common detected high-risk HPV types were HPV16,33,52,58 and 68.Among 150 patients without CA,36.67%（55） of patients were HPV positive.The proportion of multiple types HPV infection was significantly higher in female patients（16.42%,11/67） than in male patients（3.61%,3/83）（P0.01）.Conclusion The low-risk types of HPV6 and 11 were the main HPV strains in Hangzhou area.The high proportion of HPV infection was confirmed in venereal disease clinic patients with or without CA.