神经生长因子联合溴隐亭对垂体GH3细胞的抑制作用

Inhibition effects of nerve growth factor and bromocriptine on proliferation of pituitary adenoma GH3 cells

目的探讨神经生长因子（NGF）联合溴隐亭对垂体腺瘤GH3细胞增殖的影响。方法在无血清的培养条件下，将GH3细胞分成3组：对照组、溴隐亭组、NGF＋溴隐亭组，采用CCK-8法检测不同药物干预后GH3细胞的成活率。结果与对照组比较，NGF干预后GH3细胞的形态无明显改变。单独溴隐亭治疗对GH3细胞增殖的抑制作用不明显，而NGF干预后2、4、6d分别再给予溴隐亭治疗则对GH3细胞增殖均有明显抑制作用（P〈0．01），其中NGF干预后4d及6d时溴隐亭的抑制效果更明显。结论NGF能促进溴隐亭对GH3细胞增殖的抑制作用。

Objective To investigate the effects of nerve growth factor （NGF） and bromocriptine（BC）on the proliferation of pituitary adenoma GH3 cells in vitro. Methods In serum-free culture conditions, GH3 cells were divided into 3 groups： control, bromocriptine and NGF ＋ bromocriptine. The effects on proliferation of GH3 cells were tested by CCK-8 assay. Results Compared with the control group, bromocriptine alone had no inhibition effects on proliferation of GH3 cells （P〉 0.05 ）. However, proliferation of GH3 cells was effectively inhibited by bromocriptine on d2, d4, d6 after NGF treatment（P〈 0.01 ）. Conclusion NGF can enhance the inhibition effects of bromocriptine on proliferation of pituitary adenoma GH3 cell in vitro.