衍生液提取-高效液相色谱法测定食品中甲醛

HPLC Determination of Formaldehyde in Foodstuffs by Extraction with Derivative Solution

提出了高效液相色谱法测定了几种食品中甲醛含量。以衍生液2，4-二硝基苯肼乙腈溶液pH5的磷酸盐缓冲溶液（1＋1）的混合溶液为提取剂对样品进行提取，以AgilentC18色谱柱（4．6mm×250mm，5μm）为固定相，以甲醇-水（70＋30）溶液为流动相分离。甲醛的测定下限（105／N）为5．0mg·kg-1。以鲜香菇、鲜鱿鱼、奶粉和面粉样品为基体，分别添加5．0，10．0，20．0mg·kg叫甲醛标准溶液，回收率在73．3％～97．7％之间，相对标准偏差（n=6）在1．4％～5．0％之间。

HPLC was applied to the determination of formaldehyde in some foodstuffs. The formaldehyde in sample was extract with a mixture of 2,4 dinitro-phenylhydazine （dissolved in acetonitrile） and phosphate buffer solution of pH 5 （1＋1） as derivatizing solution, and separated on Agilent C18 column （4.6 mmX250 mm, 5μm）. A mixture of methanol and water mixed in the ratio of 70 to 30 （by volume） was used as mobile phase. Values of limit of determination （10S;N） found was 5.0 mg·kg-1. Using samples of fresh mushroom, fresh squid, milk powder and flour as matrixes, tests for recovery were made by standard addition method at 3 concentration levels of 5.0, 10. 0 and 20. 0 mg · kg-1, and the results obtained were in the range of 73.3%-97. 7%, with values of RSD＇s （n-6） in the range of 1.4%-5.0%.