不同条件下牡丹花粉的贮藏寿命

Storage Longevity of Tree Peony Pollen in Different Conditions

以2个牡丹品种"凤丹白"和"鲁荷红"为试材,以花粉萌发率和授粉结实率为依据,研究了室温(25℃)、4、-20、-86℃条件下的牡丹花粉贮藏寿命,以建立花粉贮藏技术。结果表明:牡丹花粉室温下保存7d、4℃下保存90d、-20℃下保存180d仍有很高的萌发率,-86℃下贮藏1a,花粉萌发率没有明显下降、授粉结实率高。室温干燥24h花粉贮藏后萌发率显著高于未干燥花粉。温度和含水量是影响牡丹花粉贮藏寿命的重要因素。自来水冲洗解冻效果最好。4℃和-86℃贮藏是解决牡丹育种花期不遇的有效措施。

Two tree peony species were used as test material, based on their germination rate and seed setting rate, the shelf life in room temperature（25℃）,4,-20 and -86℃ respectively were studied and established the pollen storage technology. The results showed pollen could be stored 7 d in room temperature, 90 d in 4℃, 180 d in -20℃, and 380 d in -86℃ ,with still high germinate rate tested by in vitro culture. The pollen germination rate maintained with high level after stored in - 86℃ for about one year, and high pollination setting rate was observed. Dry pollen displayed higher germination rate than fresh pollen after storage. As a conclusion, temperature and water content were important factors that influence pollen storage longevity. Thawing with tap water was most effective for pollen thawing. According to pollen storage longevity and florescence characteristic of peony,suggestion could be given that 4℃ or -86℃ storage was practical to resolve flowering asynchronism in tree peony breeding.