兰州百合多倍体诱导及鉴定

Polyploid Iduction and Indentification of Lilium davidii var.unicolor(Hoog) Cotton

以兰州百合鳞片为试材,利用秋水仙碱溶液对兰州百合进行了人工多倍体诱导。结果表明:秋水仙碱最佳处理浓度和最佳处理时间分别为0.1%、24h,细胞诱变率达60%。对获得的兰州百合多倍体与二倍体叶片气孔大小及密度进行测定,通过方差分析,气孔大小及密度差异达到极显著水平。对兰州百合多倍体和二倍体植株根尖染色体计数发现,二倍体植株所有细胞染色体数为2n=2x=24,多倍体植株中有的细胞染色体数为2n=2x=24,而有些细胞染色体数为2n=4x=48。

L. davidii var. unicolor（Hoog）Cotton scale was used as test material to inducted the polyploidy of L. davidii vat. unicolorr（Hoog）Cotton using eolchicine solution. The results showed that best concentration of colchcine was 0.1% in chromosome doubling induction of L. davidii vat. unicolor（Hoog）Cotton. Under the concentration,the optimial time was 24 h, and the mutagenesis rate was 60%. Comparing the size and the number of stomata per unit leaf area of the leaf of diploid and the polyploidy plants, by the statistical analysis those index was significantly different. Cytological obervation showed that the chromosome number of some cells were 2n=4x = 48, and the others were 2n =2x = 24 in polyploidy plants. In diploid plants the chromosome number of all cells were 2n=2x=24.