高碘对大鼠血清同型半胱氨酸及丙二醛的影响

Effect of Iodine Excess on Homocysteine and Malondialdehyde in Serum in Rats

目的观察不同浓度的高碘对大鼠血清中同型半胱氨酸(HCY)及丙二醛(MDA)的影响。方法将32只断乳1个月左右远交封闭群的SPF/VAF级Wistar大鼠随机分为4组,分别为正常对照(NC,水碘含量为5μg/L)组和10倍碘(10 HI,2 000μg/L)组、50倍碘(50 HI,10 000μg/L)组、100倍碘(100 HI,20 000μg/L)组,每组8只,雌雄各半。采用自由饮水方式进行染毒,连续喂养6个月。染毒结束后,测定血清同型半胱氨酸和丙二醛含量。结果与NC组相比,不论雄性还是雌性,50、100 HI组大鼠血清中HCY和MDA含量均较高,差异有统计学意义(P<0.05或P<0.01)。不同性别大鼠血清中HCY和MDA含量间比较,差异无统计学意义。结论 10倍高碘对大鼠血清中同型半胱氨酸及丙二醛未有影响,而50、100倍高碘可引起大鼠血清中同型半胱氨酸及丙二醛含量上升。

Objective To observe the effects of iodine excess on homocysteine and malondialdehyde of serum in the rat models of excessive iodine intake. Methods A total of 32 Wistar rats were randomly divided into 4 groups, 8 in each, sex ratio was 1：1. Normal control group（NC）： normal diet and normal drinking water; 10 times high iodine（HI） group： normal diet and 2 000 μg/L of iodine in drinking water;50 times HI group： normal diet and 10 000 μg/L; 100 times HI group： normal diet and 20 000 μg/L. After six months of treatment,the content of homocysteine and malondialdehyde in serum were detertnined. Results Compared with NC,the content of homocysteine and malondialdehyde significantly increased in 50 times HI group and 100 times HI group （P〈0.01）. Conclusion High iodine intake in some levels may cause homocysteine and malondialdehyde in the blood increasing in rats.