高效液相色谱-质谱联用测定人血浆中氯雷他定

Determination of loratadine in human plasma by HPLC-MS/MS

目的:建立高效液相色谱-质谱联用法测定人血浆中氯雷他定浓度。方法:分析柱为Gemini C18(5μm,50 mm×2.0mm),流动相为0.2%甲酸水溶液-甲醇(33.5∶66.5,v/v),流速:0.2 mL.min-1,柱温:25℃。采用四级杆质谱检测器电喷雾离子化(ESI)源,检测方式为正离子多离子反应监测(SRM)。结果:线性范围为0.2～25.6μg.L-1,平均提取回收率68.7%,日间RSD小于13.78%,日内RSD小于6.16%。结论:该方法快速、灵敏、重现性好,可用于临床血药浓度监测和药动学研究。

OBJECTIVE To establish a method for determination of loratadine in human plasma by HPLC-MS/MS.METHODS Using Gemini C18（5 μm,50 mm×2.0 mm）column,0.2% methanoic acid solution-methyl-alcohol（33.5：66.5）were served as mobile phase with 0.2 mL·min-1 flow rate.A surveyor TSQ quantum equipped with an at mospheric pressure ESI source was detector and was operated in positive ion SRM.RESULTS The linear ranges were 0.2-25.6 μg·L-1.The average recovery was 68.7%.The relative standard deviations of inter-day were less than 13.78% and intra-day were less than 6.16%.CONCLUSION The method is rapid,sensitive,reproducible.It was suitable for therapeutic drug monitoring and pharmacokinetic study.