多穗金粟兰营养器官的形态结构及组织化学研究

Morphological Structure of Vegetative Organs and Histochemical Localization in Chloranthus multistachys Pei

采用植物解剖学和组织化学定位方法研究多穗金粟兰(Chloranthus multistachysPei)营养器官的显微结构及萜类和黄酮类化合物在营养器官中的分布规律。结果表明:多穗金粟兰的根皮层细胞排列不整齐,液泡化明显,细胞间隙较大,有散在石细胞分布;茎皮层细胞排列不整齐,在韧皮部外方包围有少数几层束状纤维束;叶为异面叶,栅栏组织和海绵组织分化明显,叶片上、下表皮均无毛被,气孔器多为不规则型。组织化学定位结果显示萜类和黄酮类化合物分布在根的皮层、茎的皮层及韧皮部细胞中。

Anatomical and histochemical methods were used to investigate the structure of vegetative organsand the location of terpenoids and flavonoids in Chloranthus multistachys Pei.The root cortex cells showed a disordered arrangement and had larger intercellular spaces;the stem cortex cells also showed a disordered arrangement and the stem phloem was surrounded by strands of fiber;leaves had a bifacial structure,palisade tissue and sponge tissue were clearly differentiated,there were no hairs on the upper and lower epidermis of the leaf,and the stomatal apparatuses were of anomocytic type and occurred only on the lower epidermis.The histochemistry results of terpenoids and flavonoids localization were similar in the vegetative organs in Chloranthus multistachys Pei,both of which were mainly distributed in the cortex of the root,the cortex of the stem and phloem cells.