Neuritin蛋白在单眼形觉剥夺成年大鼠视皮层表达的研究

The expression of Neuritin protein in the visual cortex of monocular form deprived adult rats

目的研究正常、单眼形觉剥夺和反转缝合成年大鼠视皮层Neuritin蛋白的表达，探讨成年大鼠视皮层是否具有可塑性。方法实验研究。35只健康Wistar大鼠随机分为正常对照（N90）组，单眼形觉剥夺（MD）组和反转缝合（RS）6、12、24、48h组和RS1周组，每组5只。两种照度昼夜明暗交替时间约为12h／12h。MD组和RS组于出生后14d制作右眼形觉剥夺模型，RS组大鼠单眼形觉剥夺至90Et龄时进行反转缝合后并持续暴露于光中6h、12h、24h、48h和1周，其余两组大鼠至90日龄时直接取左侧大脑视皮层。采用免疫组织化学方法检测Neuritin蛋白在正常、单眼形觉剥夺和反转缝合成年大鼠视皮层中的表达变化。Neuritin蛋白的A值和Neuritin阳性细胞数在7组大鼠视皮层中表达的总体差异比较采用单因素方差分析，组间的多重比较采用LSD-t检验。结果N90、MD、RS6h、RS12h、RS24h、RS48h、RS1周组Neuritin蛋白平均吸光度分别为0．1097±0．0136、0．0259±0．0057、0．04751±0．0069、0．05189±0．0057、0．0649±0．0055、0．0835±0．0097、0．0845±0．0098（F=105．57，P〈0．05），染色阳性细胞数分别为163．90±5．82、142．00±3．65、150．00±5．46、152．10±5．04、156．40±5．25、156．40±6．04、155．80±6．54（F=15．39，P〈0．05）。MD组大鼠视皮层中Neuritin蛋白的表达量均低于N90组。RS6、12、24、48h组和RS1周组大鼠视皮层中Neuritin蛋白的表达量均明显高于MD组。结论Neuritin蛋白在单眼形觉剥夺成年大鼠和反转缝合不同时间大鼠视皮层表达呈动态变化，提示年龄超过视觉发育敏感期的单眼形觉剥夺成年大鼠的视皮层仍具有一定程度的可塑性。

Objective To investigate the expression of Neuritin protein in the visual cortex of normal （N90） , monocular deprived （MD） and reverse suture （RS） adult Wistar rats, explore whether the visual cortex of adult rats remains the plasticity. Methods It was an experimental research. The 35 healthy rats were divided into 7 groups, 5 in every group. The time-point for normal control and MD groups is postnatal 90 days. The time-point for RS group is 6, 12, 24, 48 h and 1 week under the RS rats were exposed to light environment. MD and RS groups were sutured right eyelid at postnatal 14 days up to postnatal 90 days. That time the right eyes were opened and the same time tarsorrhaphy was performed on the left eyes in RS groups. The visual cortex of left cerebrum in the normal control group and MD group were obtained in P80 rats, and those of RS group were acquired in corresponding time points. Expression of the Neuritin protein in the visual cortex of rats was detected by immunohistochemistry stain methods. Results Expression of Neuritin protein A of the group of N90, MD, RS 6 h, RS 12 h, RS 24 h, RS 48 h, RS lweek were 0. 1097 ±0.0136, 0.0259 ±0.0057, 0.04751 ±0.0069, 0.05189±0.0057, 0.0649 ±0.0055, 0. 0835 ±0. 0097, 0. 0845 ±0. 0098 （ F = 105.57, P 〈 0. 05 ）, the count in the visual cortex of rats were163.90±5. 82, 142.00±3. 65, 150.00 ±5.46, 152. 10 ±5.04, 156.40 ±5.25, 156.40 ±6.04, 155. 80 ± 6. 54 （F = 15.39 ,P 〈 0. 05 ）. The expression of Neuritin protein in the visual cortex of rats in the MD group was considerably lower than that of the normal control group. The expression of Neuritin protein in the visual cortex of rats in the RS 6,12,24,48 h and 1 week group was evidently higher than that in the MD group. Conclusions The dynamic change of Neuritin protein expression in the visual cortex of the MD rats and RS rats indicates that the visual cortex of adult rats with MD beyond the sensitive period of visual development remain the plasticity to some extent. It provides an experimental basis for the amblyopie treatment in elder children and adults.