白血病HL60/ADR细胞Mdr1、NF-κB的表达及解毒化瘀药的干预作用

The expressions of multidrug resistance gene 1 and NF-κB of leukemia HL60/ADR cell and the interventional function of Jiedu Huayu Chinese herbal medicine

目的探讨解毒化瘀药血清对白血病HL60/ADR细胞多药耐药基因(Mdr1)、核周因子κB(NF-κB)信号基因表达的影响。方法采用半定量逆转录聚合酶链反应(RT-PCR)法检测解毒化瘀药血清处理后HL60/ADR细胞Mdr1基因的表达;Western blot法检测NF-κB/P65、NF-κB/P50、IκBα的表达。结果解毒化瘀药血清能够降低HL60/ADR耐药细胞NF-κB/P65、NF-κB/P50I、κBα的表达,降低Mdr1耐药基因的表达。结论解毒化瘀药对HL60/ADR细胞的耐药逆转作用可能是通过阻断NF-κB信号通路,影响Mdr1耐药基因的表达,进一步逆转耐药。

Objective To discuss the influence of Jiedu Huayu Chinese herbal medicine on the expressions of multidrug resistance gene 1（Mdr1） and nuclear factor kappa B（NF-κB） of leukemia HL60/ADR cell.Methods Semi-quantitative reverse transcriptase polymerase chain reaction method was used to examine the expression of Mdr1 gene after HL60/ADR cell receiving the treatment of Jiedu Huayu Chinese herbal medicine,and Western blotting technology was applied to examine the expressions of NF-κB/P65,NF-κB/P50 and IκBα.Results The expressions of NF-κB/P65,NF-κB/P50 and IκBα and the expression of Mdr1of leukemia HL60/ADR cell could be decreased by Jiedu Huayu Chinese herbal medicine.Conclusion Jiedu Huayu Chinese herbal serum can reverse multidrug resistance in leukemia HL60/ADR cell by blocking NF-κB signal path and affecting the expression of Mdr1.