多敏灵片中咖啡因和盐酸多塞平含量的测定

HPLC determination of caffeine and doxepin hydrochloride in Duominling tablet

目的建立高效液相色谱法,测定多敏灵片中咖啡因、盐酸多塞平的含量。方法采用高效液相色谱法,以Kromasil C18（250 mm×4.6 mm,5μm）为分析柱,流动相为甲醇-水-三乙胺（75∶25∶1）,流速1 mL/min,检测波长295 nm,柱温为室温。结果咖啡因、盐酸多塞平与其他组分分离良好,且不受其他成分的干扰;咖啡因回归方程为：Y=196 604X＋218 976（r=0.999 8,n=5）,咖啡因在0.08~0.24μg的范围内线性关系良好;盐酸多塞平回归方程为：Y=60 158X＋58 230（r=0.999 9,n=5）,盐酸多塞平在0.04~0.12μg的范围内线性关系良好。方法的平均回收率分别为99.06%、96.67%,精密度（RSD）值分别为1.04%、1.13%。结论本方法操作简便、快速、准确、灵敏,适用于多敏灵片的含量测定。

Objective To establish an HPLC method for caffeine and doxepin hydrochloride determination of Duominling tablet.Methods The determination was performed on Kromasil C18（250 mm×4.6 mm,5 μm） column,the mobile phase consisted of methanol-water-triethylamine（75∶25∶1,V/V） with flow rate being 1.0 mL/min,detection wavelength at 295 nm and the temperature was normal temperature.Results Caffeine showed a good linear relationship in the range of 0.08-0.24 μg（r=0.999 8,n=5）,and doxepin hydrochloride was 0.04-0.12 μg（r=0.9999,n=5）,and the average recovery was 99.06% and 96.67% with RSD of 1.04% and 1.13%.Conclusion This method is easy,rapid,accurate and sensitive;thus it can be applied to the content determination of Duominling tablet.