冠心病痰瘀证患者载脂蛋白E基因测序检测分布

Distribution of Apo E Gene Sequencing in Patients with Coronary Heart Disease of Phlegm-stasis Syndrome

目的探讨冠心病痰瘀证与载脂蛋白E(Apo E)基因多态性的关系。方法选取冠心病患者78例,其中痰瘀证49例和非痰瘀证29例,采用全序列基因测序法检测78例冠心病患者和100名健康体检者的Apo E基因型。结果两组人群共检出5种基因型,分别是E3/3、E4/4、E2/3、E2/4、E3/4;冠心病患者E3/3基因型及ε3等位基因频率显著低于健康体检者(P<0.01),而E3/4基因型及ε4等位基因频率则显著高于健康体检者(P<0.01);冠心病痰瘀证E2/4+E3/4+E4/4基因型及ε4等位基因频率明显高于非痰瘀证(P<0.05)。结论 Apo Eε4等位基因是冠心病易患因子,与冠心病痰瘀证关系密切,推测其可能是冠心病痰瘀证的主要易感基因之一。

Objective To study the correlation between Apo E gene polymorphism and patients with coronary heart disease（CHD） of phlegm-stasis syndrome（PSS）.Methods 78 CHD patients were assigned to PSS（49 cases） and non-phlegm-stasis syndrome（NPSS）.Polymorphisms of Apo E gene in 78 CHD patients and 100 healthy subjects were detected by complete DNA sequencing.ResultsFive gene types as E3/3,E4/4,E2/3,E2/4,and E3/4 were detected in the two groups.The frequencies of genotype E3/3 and ε 3 allele were significantly lower in CHD patients than in the healthy subjects（P0.01）.But the frequencies of genotype E3/4 and ε 4 allele were significantly higher in CHD patients than in the healthy subjects（P0.01）.In CHD patients,the frequencies of genotype E2/4＋E3/4＋E4/4 and ε 4 allele were higher in PSS than in NPSS.Conclusions Apo E ε 4 allele was a susceptible allele to CHD,which was closely correlated to CHD PSS.It was inferred that it might be one of main susceptible alleles for CHD PSS.