ATP-结合相关基因mRNA在耐药性癫痫患者脑组织中的表达

Adenosine triphosphate-binding gene messenger ribonucleic acid expression in brains of drugresistant epileptics

目的研究耐药性癫痫患者脑组织中ATP-结合相关基因mRNA的表达，了解其生物学意义。方法按照病理随机法，从我们已建立的耐药性癫痫术后脑组织库（患者来自2003年6月至2004年12月期间重庆医科大学附属第一医院及广州三九脑科医院神经外科手术病人）中抽取40例脑组织标本组成试验组，对照组8例，6例来自重庆市2003年12月至2004年12月因交通事故意外死亡及其他非自然死亡后按有关法律规定需进行尸解的自然人，2例为脑出血微创手术中所取标本。在基因芯片扫描获预期结果基础上，运用RT—PCR检测ATP．结合相关的CDC2L5、ROCK1、STCH、OXSR1、ABCB6基因在耐药性癫痫患者及对照组脑组织中的表达。结果基因芯片扫描显示与ATP-结合相关的5个基因CDC2L5、ROCKI、STCH、OXSR1、ABCB6在耐药性癫痫患者脑组织中表达较对照组明显增加（CyS／Cy3比值分别为CDC2152．159，ROCK12．538，STCH2．106，OXSRI3．791，ABCB62．583），RT-PCR检测获得相同的结果。结论脑细胞中ATP一结合相关基因表达增强提示患者脑部存在能量代谢异常，其所导致的突触可塑性改变和神经元凋亡在耐药性癫痫的发生及发展中可能起着重要作用。

Objective To explore the expression of ATP （adenosine triphosphate）-binding gene mRNA （messenger ribonucleic acid） in brain tissues of patients with drug-resistant epilepsy and understand their biological characteristics. Methods The postoperative brain tissues were collected randomly from 40 patients with drug-refractory epilepsy. Based on the expected results of complementary DNA （cDNA） microarray, reverse transcription-polymerase chain reaction （RT-PCR） was used to evaluate ATP-binding gene mRNA expression in brain tissues of 40 patients with drug-resistant epilepsy and 8 normal controls. Results As compared with 8 normal control cases, the candidate genes of ATP-binding mRNA were higherly expressed in the brain tissues of patients with drug-resistant epilepsy. The rates of Cy5/Cy3 were CDC2L5 2. 159, ROCK1 2. 538, STCH 2. 106, OXSR1 3. 791 and ABCB6 2. 583 respectively. And the results of RT-PCR corresponded with those of cDNA chip. Conclusion The significant neuronal up- regulation of ATP-binding gene mRNA suggests that abnormal energy metabolism exist in brain tissues of patients with drug-resistant epilepsy. And the apoptosis of neurons and synaptic plasticity caused by abnormal energy metabolism may play an important role in the occurrence and development of patients with drug-resistant epilepsy.