摘要
目的建立大鼠慢性前脑缺血基础上的脑过度灌注模型。方法选取雄性Wistar大鼠72只依随机数字表均分为两个模型建造组。缺血模型采用双侧颈总动脉结扎,随机数字表分为空白对照、假手术组、缺血2周组、缺血4周组,每组9只,结扎前后分别测定脑额叶血流、比较各组行为学评分、脑梗死面积。过度灌注模型是在缺血模型基础上,再灌注同时经尾静脉持续输注去氧肾上腺素4μg·kg^-1·min^-1,使再灌注后脑额叶血流超过基础值的200%。随机分为空白对照组、盐水组、过度灌注0.5h组、过度灌注2h组,每组9只,再灌注前后分别测定脑额叶血流,比较各组行为学评分、血脑屏障通透性、脑干湿重比值。结果大鼠双侧颈动脉结扎后前脑血流减少可达67%4-2%,脑过度灌注组与盐水输注组的脑血流变化值差异有统计学意义(P〈0.01)。缺血2周组的神经功能评分、脑梗死面积与正常对照组差异无统计学意义,缺血4周组的脑梗死面积与正常对照组差异有统计学意义。脑过度灌注2h组的血脑屏障通透性改变有统计学意义(P〈0.05)。结论结扎大鼠双侧颈总动脉2周后脑过度灌注2h可较好地建立大鼠脑过度灌注综合征模型。
Objective To establish the cerebral hyper-perfusion model after chronic forebrain isehemia in rats. Methods A total of 72 male rats were equally randomized into 2 modeling groups. The ligation of bilateral common carotid artery could induce chronic forebrain isehemia. And 36 rats were randomly grouped by ischemia duration : control group ( n = 9 ) , sham group ( n = 9 ) , 2-week ischemia group (n =9) and 4-week ischemia group (n =9). The blood flow in frontal lobe was measured at pre- and post-ligation. The neurological score and cerebral infarction area were also compared among the groups. The cerebral reperfusion was concurrently undertaken with an infusion dose of phenylephedrine at 4 μg · kg^-1 · min^-1 via tail vein to produce cerebrally hyperperfused blood flow rate over 200% of baseline following chronic isehemia. According to cerebral hyper-perfusion duration, 36 rats were randomly assigned into 4 groups : control group ( n = 9 ), saline infusion group ( n = 9 ), 30-minute cerebral hyper-perfusion group ( n = 9) and 2-hour cerebral hyper-perfusion group ( n = 9 ). The blood flow in frontal lobe was measured before and after cerebral hyper-perfusion. The neurological score, blood-brain barrier permeability and dry- wet weight ratio of brain also were compared among the groups. Results The forebrain blood flow decreased by 67% :t:2% after the ligation of bilateral common carotid artery. There was significant difference between cerebral hyper-perfusion and saline infusion groups ( P 〈 0. 01 ). No statistic difference was observed in neurological score and cerebral infarction area between 2-week isehemia and control groups. But it was obvious between 4-week ischemia and control groups. The permeability in blood-brain barrier of rats significantly increased in 2-hour hyper-perfusion group ( P 〈 0. 05 ). Conclusion The 2-hour duration of cerebral hyper-perfusion following a 2-week ligation of bilateral common carotid artery may establish a reliable cerebral hyper-perfusion model in rats.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2011年第33期2358-2361,共4页
National Medical Journal of China
基金
首都医科大学基础-临床课题(3500-11020007)
关键词
颈总动脉
结扎术
慢性脑缺血
脑过度灌注
大鼠
模型
Common carotid artery
I,igation
Chronic cerebral isehemia
Cerebral hyperperfusion
Rats
Model