核仁素在骨肉瘤组织中的表达及其意义

Study on the expression and significance of nucleolin in osteosarcoma

目的探讨骨肉瘤中核仁素蛋白的表达情况，明确其表达与骨肉瘤临床病理遗传的关系。方法骨肉瘤组织35例，骨软骨瘤组织15例，应用免疫组化技术，检测石蜡包埋组织中核仁素蛋白的表达，并分析核仁素蛋白的表达与骨肉瘤临床病理遗传的关系。结果（1）核仁素蛋白在骨肉瘤组织中的阴性、弱、中、强表达依次为8．6％、20％、34．3％、37．1％，与骨软骨瘤组织（分别为40％，46．7％，13．3％，0）相比，其表达水平显著上调（P〈0．01）。（2）核仁素蛋白在普通型骨肉瘤中阴性、弱、中、强表达依次为6．9％、13．8％、34．5％、44．8％，与骨旁骨肉瘤（分别为16．7％，50％，33．3％，0）相比，其表达水平上调（P〈0．05）。（3）核仁素蛋白在成骨型骨肉瘤中阴性、弱、中、强表达依次为5．9％、5．9％、29．4％、58．8％，成软骨型骨肉瘤中分别为0％、33．3％、33．3％、33．3％，成纤维型骨肉瘤中分别为16．7％、16．7％、50％、16．7％，不同亚型普通骨肉瘤组织中，核仁素蛋白的表达差异无统计学意义（P〉0．05）。结论核仁素蛋白的表达与骨组织病变的性质有关，其表达水平与骨肉瘤的病理遗传有关。

Objective To investigate the expression of nucleolin in osteosarcoma and its clinical significance. Methods The expression of nucleolin was evaluated by immunohistochemistry in paraffin-em- bedded tissues from 35 cases of osteosarcoma and 15 cases of osteoehondroma. Results Among the osteo- sarcoma cases, 8.6% were negative for nucleolin, 20% weakly positive, 34. 3% moderate positive, 37.1% strong positive. The positive rate of nucleolin in osteochondroma was 40% , 46.7% , 13.3% , 0%0 , respectively. The nucleolin expression showed significant difference in the osteosarcoma and osteochondroma （ P 〈 0. 01 ）. Among the conventional osteosarcoma cases, 6. 9% were negative for nucleolin, 13.8% weakly positive, 34. 5 % moderate positive, 44. 8 % strong positive. The positive rate of nueleolin in paros- teal osteochondroma was 16. 7% , 50% , 33.3% , 0% , respectively. The nucleolin expression showed re- markable correlation with pathological and genetical classification （ P 〈 0. 05 ）. The positive rate of nucleo- lin in osteoblastic osteochondroma was 5.9%, 5.9%, 29. 4% , 58. 8% , while in chondroblastic osteoehon- droma 0% , 33.3% , 33.3%, 33.3% and 16. 7% , 16. 7% , 50%, 16.7% in fibroblastic osteochondroma, respectively. The positive expressions of nucleolin were almost the same in each subtype of osteosarcoma （ P 〉 0.05 ）. Conclusions The nucleolin expression had correlation with property of tumors in bone, and the positive expressions of nueleolin had correlation with pathological and genetic classification of osteosarcoma.