Transgelin在伴随糖尿病的胰腺癌中的表达及其对SW1990细胞运动侵袭的影响

The expression of Transgelin in pancreatic cancer accompanied with diabetes and its effects on the migration and invasion of SW1990 cell line

目的探讨骨架相关蛋白Transgelin在伴或不伴糖尿病的胰腺培组织中的表达，及其对胰腺癌SW1990细胞运动侵袭的影响。方法采用免疫组织化学法检测Transgelin在92例胰腺癌患者（其中伴精尿病者45例）的痛组织、癌旁组织（距癌边缘〉5cm）中的表达水平．分析其与临床病理特征的关系；设计并体外合成靶向Transgelin的小干扰RNA（siRNA）．将其转染到SW1990细胞中．采用Transwell小室检测转染前后细胞运动侵袭能力的改变。结果Transgelin在胰腺癌组织中的表达阳性率为68．5％（63／92），显著高于癌旁组织[33．7％（31／92），P〈0．05]。伴随糖尿病的胰腺癌组织中Transgelin表达阳性率为84．1％（38／45），显著高于无糖尿病的胰腺癌组[53．2％（25，47）．P〈0．05]。胰腺癌组织中Transgelin表达与胰腺癌的淋巴结转移及TNM分期密切相关（P〈0．05），与性别、年龄、发生部位、分化程度、门静脉或神经受侵犯无关（P〉0．05）。Transgelin siRNA干扰后48h,SW1990细胞迁移能力[穿膜缔胞数为（49．2±9．5）个]明显低于阴性对照组和空白组[（61．9±7．5）和（65．3±10．6）个，P值均〈0．05]；SW1990细咆的体外侵袭能力[穿膜细胞数为（48．0±8．6）个]也明显低于阴性对照组和空白组[（63．5±11．4）个和（67．5±9．6）个，P值均〈0．05]。结论Transgelin可能通过促进胰腺癌细胞的运动侵袭能力，参与伴随糖尿病的胰腺癌的转移发生。

Objective To investigate the expression of actin associated protein Transgelin in pancreatic cancer with or without diabetes and its effects on migration and invasion in SW1990 cell line. Methods The exprtssion of Transgelin in 92 pancreatic cancer tissue specimens （I5 cases accompanied with diabetes） and adjacent tumor free tissue specimens （over 5cm from the edge of the tumor） was detected by immunohislochemistry, and their association with clinical pathological characteristics were also analyzed. Transgelin siRNA was designed and transfected into pancreatic cancer cell line SW1990. The changes of migration and invasion before and after transfeetion were observed through Trauswell test. Results The positive percentage of Transgelin expression in pancreatic cancer was 68.5%（63 /92）, which was significantly higher than that of adjacent tumor free tissues [33.7%（31/92）,P〈0. 05]. The positive percentage of Transgelin expression in pancreatic cancer accompanied with diabetes was 84. 4% （38/45）, which was significantly higher than that without diabetes [53. 2% （25/47）,P〈0. 05], The expression of Transgelin in pancreatic cancer tissues was associated with lymph nodes metastasis and TNM staging （both P〈0. 05）, but not related with gender, age, site, differentiation and portal vein or nerve invasion （P〉0.05）. After Transgelin was interfered for 48 hours, the migration ability was signlficantly lower （migration cell number 49.2 ±9.5 cells） than negative control group （61.9±7.5 cells） and blank group （65.34±10.6 cells） （both P〈0.05） , and the invasion of SW1990 cells （48.0±8.6 ceils） also significantly lower than negative control group （63.5±11.4 cells） and blank group （67.5±9.6 cells） （both P〈0.05）. Conclusion Transgelin may involve in the metastasis of pancreatic cancer accompanied with diabetes through promoting pancreatic cancer cell migration and invasion.