心肺复苏大鼠脑红蛋白缺氧诱导因子-1α的表达变化

Expression of Ngb and HIF -1α in brain tissue of postresuscitation rats

目的研究大鼠心肺复苏后不同时间点脑红蛋白（neuroglobin，Ngb）、缺氧诱导因子-1α（HIF-1α）在mRNA水平表达中的变化规律，探讨Ngb在心脏骤停后脑缺血缺氧损伤病理变化中的作用机制及其诱导表达途径。方法SD雄性大鼠40只随机分为五组（n=8）：假手术组及复苏后1h、6h、12h、24h组。窒息法致大鼠心脏骤停后予以心肺复苏，复苏后不同时间点处死大鼠，应用RT—PCR法观察脑组织NgbmRNA、HIF-1αmRNA表达情况。结果复苏组脑组织NgbmRNA表达高于假手术组（P〈0．05），并于损伤后12h达高峰；与假手术组比较，复苏组脑组织HIF-1αmRNA表达增加（P〈0．05），且于复苏后1h、6h、12h、24h呈持续上升趋势。结论大鼠心脏骤停后早期脑组织NgbmRNA、HIF—lotmRNA表达增加，且呈时相性改变。

Objective To investigate the expression of neuroglobin （Ngb）, hypoxia - inducible factor （HIF） -1α in mRNA level at different time points, which can help us understand the role of Ngb in the pathogenesis of cerebral hypoxia - ischemia after cardiac arrest. Methods 40 SD rats were divided into five groups randomly （n = 8）：sham operation group, 1, 6, 12, 24 h group after resuscitation. The rats model of cardiac arrest were duplicated by asphyxia method ; Reverse transcription polymerase chain reaction applied to observe the changes of mRNA expression of Ngb and HIF - 1α in brain tissue. Results The mRNA expression of Ngb was significantly higher in resuscitation group than in sham group （P 〈0.05）, and reached the peak at 12 h; The mRNA expression of HIF- 1α was significantly higher in resuscitation group than in sham group （ P 〈 0.05 ） and kept increasing at 1, 6, 12 till 24 h. Conclusion The expression of Ngb and HIF - 1α increased in brain tissue of cardiac arrest rats and changed with time.