脓毒血症患者外周血中Th17细胞检测及意义

Significance of Th17 cells detection in peripheral blood of patients with sepsis

目的探讨脓毒症患者外周血Th17细胞和细胞因子IL-17、IL-6的表达水平及其对免疫功能的影响。方法选取2010—04～2010—11住我院ICU病房的脓毒症患者62例，采用流式细胞术检测外周血Th17％（Th17／CD4＋T），应用酶联免疫吸附方法（ELISA）检测血浆IL-17和IL-6的表达水平。同时选取来自我院查体中心的健康查体者30例作为对照组。结果脓毒症患者外周血Th17％明显高于健康对照组（P〈0．01）；血浆IL-17、IL-6的表达亦显著高于对照组。患者血浆IL-17与IL-6表达水平呈正相关（r=0．886，P〈0．01）。Th17的表达与IL-17、IL-6的表达亦呈正相关（r=0．846，r2=0．891，P均〈0．01）。结论脓毒血症患者外周血中Th17％增加，血浆IL—17及IL-6表达水平亦升高，提示Th17参与脓毒血症的发病。

Objective To investigate the Thl7 and its associated cytokines IL- 17 and IL -6 in patients with sepsis, and their impact on immune function. Methods Sixty - two patients admitted to ICU of our hospital in April 2010 to November 2010. The frequencies of Thl7 in peripheral blood were detected by flow cytometric analysis. Thl7 related plasma cytokines （ including IL - 17 and IL - 6 ） concentrations were measured by enzyme linked immuno - sorbent assay. Thirty healthy individuals came from the examination center of our hospital were served as controls. Results The frequencies of Thl 7 in peripheral blood was significant higher in septic patients than in normal controls （ P 〈 0. 01 ）. Th17 related cytokines IL - 17and IL - 6 markedly increased in patients with sepsis compared with normal controls. Plasma concentration of IL - 17 was positively correlated with concentration of IL - 6 （ r = 0. 886,P 〈 0.01 ）. Significant correlation was found between Th17 and IL - 17, IL - 6 （ r1 = 0. 846, r2 = 0. 891 ,both P 〈 0. 01 ）. Conclusion The frequencies of Thl7 increase in patients with sepsis, and its related cytokines IL - 17 and IL -6 also increase, which suggest Th17 may play a potential role in paiients of sepsis.