血液中N-甲基氨甲酰加合物作为二甲基甲酰胺职业接触标志物可行性探讨

Feasibility Study on Taking Blood N-methylcarbamoyl Adduct as an Occupational Exposure Biomarker of DMF

目的探讨血液中N-甲基氨甲酰加合物作为二甲基甲酰胺(DMF)职业接触标志物可行性。方法选择不同生产部门、不同DMF接触水平的职业人群87人,不接触DMF等有机溶剂的人员22人作为对照。气相色谱-质谱法测定N-甲基氨甲酰加合物经过一系列降解后的产物3-甲基-异丙基乙内酸脲(MVH)浓度。结果各类DMF职业接触人员血红蛋白中均检出了不同含量的MVH,而对照人群均未检出。血液中N-甲基氨甲酰加合物水平与DMF接触情况呈正相关。结论血液中N-甲基氨甲酰加合物有望成为反映DMF长期接触的标志物。

Objective To explore the feasibility of taking blood N-methylcarbamoyl adduct as an occupational exposure biomarker of DMF.Methods 87 workers occupationally exposed to DMF were taken as the experiment subjects.22 staff unexposed to DMF or any other organic solvents were taken as the controls.Concentration of MVH,the degeneration product of N-methylcarbamoyl adduct,was determined by GC-MS.Results In hemoglobin of DMF exposed workers,MVH in different concentrations was detected,but not in any one of the controls.Concentration of blood N-methylcarbamoyl adduct was positively correlated with DMF exposure levels.Conclusions Blood N-methylcarbamoyl adduct might be considered as a new occupational exposure biomarker of DMF.