何首乌饮对衰老大鼠卵巢组织凋亡相关蛋白Bax/Bcl-2及Caspase-3表达的影响

Effect of the Heshouwu-yin on expression of apoptosis-associated Bax/Bcl-2 and Caspase-3 protein in ovary of aging rats

目的:探讨何首乌饮对衰老大鼠卵巢组织凋亡相关蛋白Bax/Bcl-2及Caspase-3表达的影响。方法:D-半乳糖连续腹腔注射致亚急性衰老动物模型,造模后灌胃给予何首乌饮,连续60 d,大鼠断头取卵巢。采用免疫组织化学分析方法检测各组大鼠凋亡相关基因Bcl-2和Bax的表达;Western blotting法检测各组大鼠卵巢Caspase-3蛋白表达的变化。结果:模型组较正常组卵巢细胞Bax和Caspase-3表达量增加,Bcl-2表达减弱;何首乌饮可使卵巢Bax和Caspase-3表达量降低,Bcl-2表达增加,并呈现一定的剂量依赖性,差异有统计学意义(P<0.05)。结论:何首乌饮方剂能延缓衰老大鼠卵巢细胞凋亡的发生,其机制可能是通过干预凋亡相关蛋白Bax/Bcl-2及Caspase-3的表达抑制卵巢细胞的凋亡。

Objective： To investigate the effect of Heshouwu-yin on ovary apoptosis and expression of the related Bax/Bcl-2 and Caspase-3 in aging rats. Methods： Sub-acute aging was induced by continually intraperito- neous D-gal injection in rats. After oral lavage of Heshouwu-yin, the expression of apoptosis-related Bax/Bcl-2 and Caspase-3 were detected by immunocytoehemistry and Western blotting. Results： The expression of Bax and Caspase-3 increased, and Bcl-2 expression were decreased in aging rats. Heshouwu-yin reduced the expression of Bax and Caspase-3, and increased Bcl-2 expression in ovary （P 〈 0.05） in a dose-dependent manner. The high dose exhibited the maximal effect. Conclusion： Heshouwu-yin can reduce the incidence of ovary apoptosis, and its anti-aging effect may associated with suppressing the expression of Bax/Bcl-2 and Caspase-3, and inhibiting apoptosis.