麻黄汤等超微饮片与传统饮片对斑马鱼胚胎毒性的比较研究

Comparative Study of Embryotoxicity to Zebrafish Induced by Chinese Herbal Prescriptions Composed of Ultrafine Powder Herbs or Traditionally Processed Herbs

目的比较麻黄汤等三个中医经典方剂超微饮片与传统饮片水煎液对斑马鱼胚胎毒性的影响,为其超微饮片的临床安全应用提供科学依据。方法采用模式生物斑马鱼发育正常的胚胎,在不同药物浓度药物环境中培养72 h后,于倒置显微镜下观察胚胎的存活、发育、致畸情况,72 h后,计算致斑马鱼胚胎半数死亡浓度(LC50)。结果麻黄汤超微饮片组与传统饮片组LC50分别为4.641,7.569 mg/mL;葛根芩连汤超微饮片组与传统饮片组的LC50分别为1.473,3.570 mg/mL;小柴胡汤超微饮片组与传统饮片组的LC50分别为2.223,2.441mg/mL。结论斑马鱼胚胎毒性分析结果表明,相同药物和相同浓度时,超微中药饮片毒性大于传统中药饮片。

Objective To compare the embryotoxicity to zebrafish induced by classic Chinese herbal prescriptions composed of ultrafine powder herbs or traditionally processed herbs, thus to provide scientific basis of clinical use. Methods We observed the survival, development and teratogenesis of zebrafish embryo living in different concentra tions of herbal medicine under inverted microscope, and then calculated median lethal concentration（LC50） for zebrafish after 72 h. Results The LC50 of ultrafine-powder Mahuang Decoction was 4.641 mg/mL while that of traditionally-processed Mahuang Decoction was 7.596 mg/mL. The LC50 of uhrafine-powder Gegen Qin Lian Decoction was 1.473 mg/mL and that of traditionally-processed Gegen Qin Lian Decoction was 3.570 mg/mL. The LC50 of uhrafinepowder Xiao Chaihu Decoction was 2.223 mg/mL and that of traditionally-processed Xiao Chaihu Decoction was 2.441 mg/mL. Conclusion It is indicated that embryotoxicity to medicine is stronger than that induced by traditionally-processed