丹参总提物抗运动性疲劳作用研究

Effect of total extracts from Salvia miltiorrhiza on sports fatigue

目的:观察丹参总提物(DSH)对小鼠抗运动性疲劳作用。方法:采用转轮式疲劳仪制备小鼠疲劳模型,测定运动力竭时间、血乳酸(LD)、血尿素氮(BUN)和肝组织中肝糖元(HG)含量,观察丹参总提物的抗疲劳作用;测定血清乳酸脱氢酶(LDH)活性、全血中血红蛋白浓度(Hb)以及氧化损伤指标骨骼肌丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性,探讨丹参总提物抗疲劳作用的相关机制。结果:DSH可明显延长实验小鼠运动力竭时间,显著降低小鼠运动后血清LD和BUN含量,提高小鼠血清LDH活性,显著增加HG的储备量和降低血清MDA含量。结论:DSH具有明显的抗疲劳作用,其抗疲劳机制可能与增加乳酸脱氢酶活性、肝糖元储备以及抗氧化作用有关。

Objective：To explore the anti-fatigue effect of the total extract of Salvia miltiorrhiza Bunge（DSH） on endurance training mice.Methods：The exhausting time of swiming mice were tested in a turning-fatigue tester.After the mice exhausting,the urea nitrogen（BUN）,lactic acid（LD）,LDH activity in serum,hemoglobin in whole blood（Hb）,hepatic glycogen（HG） in liver,MDA and SOD activity in bone,were measured.The anti-fatigue effect of DSH and the mechanism were discussed.Results：Exhausting swimming test showed that the swimming time in a DSH group was higher than that in the control group.The content of LD,BUN,MDA was lower,the content of HG and activity of LDH were higher than the control group.Conclusions：DSH showed obvious anti-fatigue effect,which can attribute to increase the activity of LDH and the content of HG,and its antioxidation in-vivo.