COM.TEC型血细胞分离机行外周血单个核细胞采集前后患者血液指标变化的比较

Changes of haematology parameter in patients with cancer before and after collecting peripheral blood mononuclear cell with COM. TEC blood cell separator

目的观察COM.TEC型血细胞分离机MNC程序行外周血单个核细胞采集前后患者血液学指标的变化。方法用COM.TEC型血细胞分离机MNC程序采集54例肿瘤患者外周血单个核细胞,行高效细胞毒性T细胞治疗(ECTL)的患者每次处理抗凝全血(2055±342)ml,抗凝剂(225±27)ml,时间为(57±13)min。行细胞因子诱导的杀伤细胞(CIK)治疗的患者每次处理抗凝全血(1776±206)ml,抗凝剂(209±27)ml,时间为(49±15)min。结果单采后患者白细胞(WBC)、血小板(PLT)、血红蛋白(Hb)、红细胞比容(HCT)与采集前比较有所下降(P<0.05),粒细胞计数与采集前比较无变化(P>0.05),采集过程中未发生严重不良反应。结论采集后的WBC、PLT、Hb、HCT虽然分别有所下降,但均在正常值的范围内,且COM.TEC型血细胞分离机MNC程序能采集到足够数量单个核细胞供肿瘤免疫治疗,采集方法可靠,可以满足临床治疗的要求。

Objective To observe the changes of haematology parameter in patients with cancer before and after collection of peripheral blood mononuclear cell with COM. TEC blood cell separator using MNC program. Methods Using COM. TEC blood cell separator MNC program, the peripheral blood mononuclear cells were collected in 54 patients with cancer. In pat/ents treated by ECTL each processing anticoagu/ation whole blood was （2055±342） ml, anticoagulants was （225±27） ml, time was （57 ± 13） min. In patients treated by CIK each processing anticoagulation whole blood was （1776±206） ml, anticoagulants （209±27） ml, （49± time for 15） min. Results After collection WBC, platelet （ PLT）, hemoglobin （Hb） , hematocrit （Hct） decreased, compared with before collecting, the differences had statistical significance （P 〈 0.05 ）. No serious adverse reactions happened during the collecting process. Conclusion Although WBC, PLT, Hb, Hct declined after collection, but the changes were respectively in the normal range, and COM. TEC blood cell separator using MNC program can collect enough number of peripheral blood mononuclear cell for cancer immunotherapy. This sampling method is reliable, can satisfy the requirements of clinical treatment.