摘要
建立了高效液相色谱法直接测定榨菜中的防腐剂、甜味剂、合成着色剂的方法。采用C18柱以甲醇-乙酸铵(0.02 mol/L)为流动相,紫外检测波长为230、254 nm,可在25 min内将苯甲酸、山梨酸、脱氢乙酸、对羟基苯甲酸乙酯、对羟基苯甲酸丙酯、糖精钠、乙酰磺胺酸钾(安赛蜜)、天门冬酰苯丙胺酸甲酯(阿斯巴甜)、柠檬黄、日落黄完全分离,测定结果的相对标准偏差为1.1%～3.3%(n=3),回收率为96.6%～99.4%。
A method was developed for simultaneous determination of food additives in pickled mustard by high performance liquid chromatography （ HPLC ） directly. The analysis was performed on a C18 column using methanol - ammonium acetate （ 0.02 tool/L） as mobile phase, and ultraviolet detection wavelength was 230, 254 nm. Benzoic acid, sorbic acid, dehydroaeetic acetate, ethyl p-hydroxy benzoate, propyl p-hydroxyl benzoate, saccharin sodium, acesulfame potassium （acesulfame-K） , L-aspartyl-L-phenyl- alanine methyl ester （aspartame） , tartrazine and sunset yellow were separated completely in 25 minutes. The RSD of determination re- suits were in the range of 1.1% - 3.3% （ n = 3 ） , and the recoveries were in the range of 96.6% - 99.4%.
出处
《化学分析计量》
CAS
2011年第5期20-22,共3页
Chemical Analysis And Meterage
基金
浙江省分析测试科技计划项目(2007F70073)
关键词
高效液相色谱
榨菜
食品添加剂
high performance liquid chromatography （[-IPLC） , pickled mustard, food additive
