柱前衍生化-高效液相色谱法分析木糖结晶母液的单糖组成

ANALYSIS OF MONOSACCHARIDE COMPOSITION IN CRYSTALLIZATION SOLUTION OF XYLOSE BY PRECOLUMN DERIVATIZATION-HPLC

采用1-苯基-3-甲基-5-吡唑啉酮(PMP)柱前衍生化-反相高效液相色谱(HPLC)法建立了8种常见单糖的分离模式,并用于木糖结晶母液单糖组成的定量分析。结果表明:木糖结晶母液至少由甘露糖、鼠李糖、纤维二糖、葡萄糖、半乳糖、木糖、阿拉伯糖及岩藻糖8种单糖组成,其中以葡萄糖、半乳糖、木糖和阿拉伯糖为主。以峰高定量,8种单糖的浓度与相应的峰高呈良好的线性关系,相关系数r2在0.9897～0.9994范围内,方法检出限为0.0021～0.003 1 mg/mL,回收率为98.4%～101.0%,测定结果的相对标准偏差为0.66%～1.21%(n=6)。该方法简单、快速、灵敏,可用于木糖结晶母液的质量控制。

The reversed - phase high performance liquid chromatographic （HPLC） method of pre-column - derivatization with 1-phenyl-3-methyl-5-pyrazolone （PMP） was developed to separate the traditional eight monosaccharides and detect the monosaccharide composition of crystallization solution of xylose. The results showed that crystallization solution of xylose consisted of mannose, rhamnose, cellobiose, glucose, galactose, xylose, arabinose, fucose, and the dominant component monosaccharides were glucose, ga- lactose, xylose and arabinose. Peak height was used to quantify. The concentration of eight kinds of monosaccharide was liner with cor- respond peak area with correlation coefficients（ r2） of 0.989 7 -0. 999 4. The detection limit was 0. 002 1 -0.003 1 mg/mL. The re- covery was 98.4% -101.0%, and relative standard deviation of detection results was 0.66% -0.21% （n = 6）. The HPLC method is simple, rapid, sensitive, convenient and can be applied to the quality control of crytallization solution of xylose.