植物乳杆菌细胞壁肽聚糖的微胶囊化

Microcapsulation of Cytoderm Peptidoglycan Derived from Lactobacillus plantarum

以壳聚糖(CS)为壁材,采用乳化交联法制备载植物乳杆菌细胞壁肽聚糖(PG)的CS微球,并通过动物实验研究口服PG微球的缓释效果。通过低、中、高3种剂量裸PG和PG微球对小鼠进行灌胃1周处理,每隔3d断尾取血,连续进行15d,ELISA法检测小鼠血清中溶菌酶含量。结果表明:微球的载药量和包封率依壁、芯材比而变化,其中,PG和CS质量比为1:1的复合微球平均载药量为(37.00±3.24)g/100g,包封率为(92.00±2.38)%。在免疫后第9天各微球组小鼠血清中溶菌酶含量均达到最高值,明显高于对照组,免疫后第15天各微球组血清中溶菌酶含量仍保持较高水平。载PG的CS微球具有显著的缓释效果,能够显著延长PG作用时间。

Chitosan microspheres encapsulating cytoderm peptidoglycan derived from Lactobacillus plantarum were prepared by emulsion-crosslinking method.The sustained-release effect of peptidoglycan was evaluated in experimental mice orally administered with naked peptidoglycan or peptidoglycan microspheres for a consecutive week at low,medium and high dosages.Blood was collected from mouse tails within 15 d at an interval of 3 d.Enzyme-linked immunosorbent assay（ELISA） was used to measure lysozyme content in mouse serum.The results showed that the average drug loading capacity（LC） and encapsulation efficiency（EE） of microspheres varied with different ratios between peptidoglycan and chitosan.At the ratio of 1：1,the LC and EE were（37.00 ± 3.24） g/ 100 g and（92.00 ± 2.38）%,respectively.The highest lysozyme content in serum was achieved at the 9th day post-administration of peptidoglycan microspheres,which was significantly higher than that in the control group.Meanwhile,high lysozyme content could last to the 15th day post-vaccination.Therefore,chitosan microspheres revealed an obvious sustained-release effect,which can effectively extend the action time of peptidoglycan.