产核糖核酸酶PA110-7菌株的筛选与初步鉴定

Isolation and Primary Identification of Ribonuclease-producing Strain PA 110-7 from Suaedae glaucae Bunge

为筛选产核糖核酸酶并且具有抗病毒活性的植物来源的菌株,并鉴定。本实验从健康的碱蓬中分离到50株益生菌,通过平板显色法筛选得到9株产核糖核酸酶的益生菌,通过噬菌斑法进行复筛得到抗噬菌体率高达69%的菌株110-7。根据菌株的菌落形态、大小、颜色,革兰氏染色、芽孢染色等观察菌体形态以及生理生化实验、16S rDNA序列测定进行鉴定,初步判定该菌株为苏云金芽孢杆菌(Bacillus thuringiensis)。

In order to screen and identify ribonuclease-producing strain and evaluate its antiviral activity,50 probiotics from healthy Suaedae glaucae Bunge were isolated and 9 ribonuclease-producing strains were screened by color plate method.The inhibitory activity of strain PA110-7 against Escherichia coli was 69%.Based on the analyses of colony morphology,size and color,Gram staining,spore staining,physiological-biochemical characterization and 16S rDNA sequencing,strain PA110-7 was identified as Bacillus thuringiensis.