副干酪乳杆菌乳酸脱氢酶的生物信息学分析

Bioinformatic Analysis of Genes Encoding Lactate Dehydrogenase from Lactobacillus paracasei

目的:分析和预测副干酪乳杆菌(Lactobacillus paracasei)乳酸脱氢酶(lactate dehydrogenase,LDH)基因及其编码蛋白的结构和特性,以指导其生物学功能的实验研究。方法:利用美国国家生物技术信息中心(NCBI)和瑞士生物信息学研究所的蛋白分析专家系统(ExPASY)中有关基因和蛋白的序列和结构信息分析的各种工具,结合其他生物信息学分析软件,如ClustalX、VMD,从数据库中得到乳酸脱氢酶基因,分析、预测该基因编码的蛋白理化性质、翻译后的修饰位点、拓扑结构、二级结构、三级结构和功能域。并与其他微生物的乳酸脱氢酶蛋白序列进行比对,得出它们的保守序列。结果:该基因编码335个氨基酸,其蛋白理论分子质量为36608.8u,预测该蛋白有3个跨膜区。与干酪乳杆菌的乳酸脱氢酶进化关系最近。结论:应用生物信息方法可预测得到副干酪乳杆菌的乳酸脱氢酶的结构与功能方面的信息。

Objective： To analyze and predict the structure and characteristics of lactate dehydrogenase from Lactobacillus paracasei for guiding experimental studies of its biological functions and application.Methods： Bioinformatic tools provided by the NCBI website and ExPasy as well as bioinformatic software packages such as ClustalX and VMD were used to identify sequence encoding LDH protein from database.The characteristics including physical-chemical characteristics,post-translational modification sites,topological structure,secondary structures,tertiary structure and domains of the deduced protein were analyzed.The sequences encoding LDH from Lactobacillus paracasei and other microorganisms were compared to obtain the conserved sequence.Results： The gene codes for 335 amino acids were achieved.The theoretical molecular weight of the deduced protein was 36608.8 u.The encoding protein was elucidated to have 3 trans-membrane regions.Through the comparison of amino acid sequences,LDH from Lactobacillus paracasei had high homology with LDH from Lactobacillus casei.Conclusion The structure of ldh gene and LDH protein from Lactobacillus paracasei can be bioinformatically analyzed and predicted.