抗氧化活性林蛙胎卵多肽酶解酶的筛选

Enzyme Screening for Enzymatic Preparation of Rana chensinensis Egg Polypeptide with Antioxidant Activity

以东北林蛙胎卵为原料,以酶解液对DPPH自由基和羟自由基(.OH)清除率为指标,分别研究木瓜蛋白酶、碱性蛋白酶、中性蛋白酶、复合蛋白酶和胰蛋白酶在最佳酶作用条件下酶解时间对酶解液清除DPPH自由基、.OH的影响,筛选出适合生产抗氧化活性林蛙胎卵蛋白肽的碱性蛋白酶、木瓜蛋白酶、中性蛋白酶,再经过复合双酶酶解林蛙胎卵实验,结果表明,以中性蛋白酶与碱性蛋白酶复合酶解林蛙胎卵3h得到肽液的.OH清除率与DPPH自由基清除率最高,达到80.91%和70.45%。

Rana chensinensis eggs were used as the materials to explore the effects of papain,alkaline protease,trypsin,neutral protease and protamex under their optimal action conditions on radical scavenging capacity of Rana chensinensis egg hydrolysates.The bioactive polypeptide with antioxidant activity was screened through the evaluation of scavenging capability against DPPH and hydroxyl free radicals.The results indicated that the polypeptide generated from 3 h digestion of Rana chensinensis eggs by alkaline protease combined with neutral protease revealed the highest scavenging capacity against hydroxyl and DPPH free radicals,which were 80.91% and 70.45%,respectively.