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冷却肉中假单胞菌16S rRNA基因不同可变区片段DGGE研究 被引量：1

DGGE Analysis of Different Variable Region Fragments in 16S rRNA Gene of Pseudomonas from Chilled Meat
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摘要 为揭示变性梯度凝胶电泳(DGGE)技术存在的一些技术缺陷,本实验以冷却肉中不同假单胞菌相关克隆为例,分别在V3和V6～V8两个不同可变区片段比较不同假单胞菌的16S rRNA基因差异。结果表明,以不同可变区为扩增目标进行研究,假单胞菌的DGGE图谱结果存在差异;基因片段序列的G+C含量与图谱中迁移位置并非绝对相关。DGGE技术的这一缺陷很大程度上是由细菌16S rRNA基因序列的自身特性所引起的。 In this study,16S rRNA gene sequences of different Pseudomonas cloned from chilled meat were analyzed based on V3 and V6-V8 region fragments to explore the technical defects of denaturing gradient gel electrophoresis（DGGE）.The results showed that different variable regions of 16S rRNA could lead to different DGGE results,and the（G ＋ C） contents of variable region fragments were not related to the migrating distance in the DGGE.Therefore,the limitation of DGGE was due to the innate characteristics of bacterial 16S rRNA gene sequences to some extent.
作者 江芸 高峰 苏勇 徐幸莲 周光宏
机构地区 南京师范大学金陵女子学院食品科学与营养系 南京农业大学国家肉品质量安全控制工程技术研究中心 南京农业大学动物科技学院
出处 《食品科学》 EI CAS CSCD 北大核心 2011年第17期287-291,共5页 Food Science
基金 国家自然科学基金项目(31071614) 江苏省高校自然科学基础研究项目(08KJB550005)
关键词 16S RRNA基因 变性梯度凝胶电泳 假单胞菌 16S rRNA gene denaturing gradient gel electrophoresis Pseudomonas
分类号 TS251.51 [轻工技术与工程—农产品加工及贮藏工程]
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参考文献28

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同被引文献14

  • 1周彦玢,束蓉,刘大力.不同16S rDNA通用引物对DGGE进行牙周微生物群落分析的影响[J].上海交通大学学报（医学版）,2011,31(5):684-687. 被引量：3
  • 2龙良鲲,羊宋贞,姚青,朱红惠.AM真菌DNA的提取与PCR-DGGE分析[J].菌物学报,2005,24(4):564-569. 被引量：50
  • 3邢德峰,任南琪,宋佳秀,曲敏,徐香玲.不同16SrDNA靶序列对DGGE分析活性污泥群落的影响[J].环境科学,2006,27(7):1424-1428. 被引量：66
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  • 5Lti XC, Weng X, Zhang W, et al. Microbial diversity of traditional fermentation starters for Hong Qu glutinous rice wine as determined by PCR-mediated DGGE[J]. Food Control, 2012, 28(2): 426-434.
  • 6Calabria de A J, Schneider RP. DGGE with genomic DNA: suitable for detection of numerically important organisms but not for identification of the most abundant organisms[J]. Water Research, 2008, 42(20): 5002-5010.
  • 7Kim TW, Lee JH, Kim SE, et al. Analysis of microbial communities in doenjang, a Korean fermented soybean paste, using nested PCR-denaturing gradient gel electrophoresis[J], International Journal of Food Microbiology, 2009, 131(2): 265-271.
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食品科学
2011年 第17期

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