可溶性膳食纤维对高脂膳食大鼠血清短链脂肪酸的影响

Effects of Soluble Dietary Fibers on Serum Short-chain Fatty Acids of High-fat-diet Rats

以SD大鼠为实验动物,饲喂高脂饲料,同时灌胃可溶性的燕麦β-葡聚糖、番茄皮膳食纤维和葡萄皮膳食纤维,喂养50d后取血,以中空纤维支撑液膜萃取-气相色谱法测定血脂实验大鼠血清的短链脂肪酸(short-chainfatty acids,SCFA)含量,探讨3种可溶性膳食纤维对高脂膳食大鼠血清SCFA的影响。研究发现,与高脂膳食模型大鼠相比,燕麦β-葡聚糖对血清丙酸、丁酸含量有显著降低作用(P<0.01),分别下降了76.09%和55.79%;与高脂膳食模型大鼠相比,番茄皮膳食纤维组大鼠的血清乙酸含量有显著性提高(P<0.01),提高了81.56%,对血清戊酸含量有显著降低作用(P<0.01),下降了62.63%;与高脂膳食模型大鼠相比,葡萄皮膳食纤维对血清乙酸含量有显著提高作用(P<0.01),提高了97.60%,但对血清异丁酸、异戊酸、戊酸有显著降低作用(P<0.01),其分别下降了66.59%、39.33%和63.82%。

In order to explore the effects of soluble dietary fibers on serum short-chain fatty acids（SCFA） of SD rats fed high fat diet,the rats were orally administered with soluble oat β-glucan,soluble tomato skin dietary fiber and soluble grape skin dietary fiber for 50 consecutive days.The serum SCFAs of rats were extracted by hollow fiber-supported liquid membrane extraction and quantitatively determined by gas chromatography.Compared with the model rats,soluble oat β-glucan could result in a significant decrease of propionate acid and n-butyric acid by 76.09% and 55.79%,respectively（P〈0.01）;soluble dietary fiber from tomato peel could result in an increase of acetic acid by 81.56%（P〈0.01） and a significant reduction of n-valeric acid by 62.63%（P〈0.01）;soluble dietary fiber from grape peel could significantly increase acetic acid by 97.60%（P〈0.01） and significantly reduce i-butyric acid,i-valeric acid and n-valeric acid by 66.59%,39.33% and 63.82%,respectively（P〈0.01）.