红酵母RY-08产类胡萝卜素的发酵条件研究

Studies on Fermentation Conditions of Carotenoid Produced by Rhodotorula sp. RY-08

研究不同碳源和氮源对红酵母RY-08产类胡萝卜素的影响,并用正交试验对碳源、氮源、装液量及发酵时间进行优化。结果表明在葡萄糖40 g/L,蛋白胨20 g/L,装液量30 mL/250 mL,发酵时间72 h的发酵条件下其生物量、类胡萝卜素含量和产量分别达到19.7 g/L,418.6μg/g,8.2 mg/L。

Effects of carbon and nitrogen source on carotenoid production of Rhodotorula sp.RY-08 were investigated,and the conditions for carotenoid fermentation were optimized by orthogonal test method.It indicated that the cell biomass,carotenoid content and yield from strain RY-08 could respectively reach 19.7 g/L,418.6 μg/g and 8.2 mg/L under the fermentation conditions： glucose 40 g/L,tryptone 20 g/L,medium 30 mL（in 250 mL flask）,and culture time 72 h.