灵芝三萜酸分批发酵的非结构动力学模型

Unstructured kinetic models for triterpene acids of Ganoderma lucidum in batch fermentation

研究了灵芝胞内和胞外三萜酸在30L发酵罐中分批发酵的动力学特征。利用Sigmoid函数构建了灵芝细胞生长、底物消耗、胞内和胞外三萜酸的非结构动力学模型,并根据Boltzmann拟合求解出各模型参数。结果表明,各模型预测值能够较好地吻合实验实测值。灵芝细胞比生长速率在第2.5天达到最大值(μmax),为0.700d-1;葡萄糖比消耗速率在第2.4天达到最大值(qS,max)为1.060d-1;胞内三萜酸比合成速率在第4.7天达到最大值(qITA,max)为11.345mg/(g·d);胞外三萜酸比合成速率在第5天达到最大值(qETA,max)为10.077mg/(g·d)。灵芝胞内外三萜酸的合成和细胞生长均呈现部分偶联关系。Sigmoid模型在灵芝真菌生长和胞内外三萜酸形成规律的拟合方面有较好的适应性,研究结果为分批发酵法生产灵芝三萜酸提供了理论依据。

The dynamic behavior of intracellular triterpene acids（ITA） and extracellular triterpene acids（ETA） of Ganoderma lucidum by batch fermentation in a 30L stirred tank bioreactor was observed.Unstructured kinetics of cell growth,glucose comsumption,ITA and ETA were investigated based on Sigmoid model.The parameters of the model were determined by Boltzmann simulation,and the values predicted by the models matched well with those obtained from the observed.The maximum rate for specific cell growth was found to be 0.700d-1,at day 2.5（μmax）,specific glucose consumption rate（qS,max）,1.060d？1 at day 2.4 specific ITA production rate 11.345mg/（g·d） at day 4.7（qITA,max） and the maximum specific ETA production rate（qITA,max） 10.077mg/（g？d） at day 5.Both ITA and ETA production were partially associated with cell growth,and the unstructured Sigmoid models for cell growth and ITA and ETA production showed good accordance with the experimental data.