摘要
在来源于香菇Lentinula edodes不同双核菌株的单核菌株间进行单-单杂交,并利用双向核迁移现象获得两对双核菌株。之后采用挑取菌落尖端单根菌丝的方法纯化所获得的双核菌株并通过双-单杂交进行单向核迁移,进一步纯化所获得的双核菌株。从菌落形态、显微镜镜检、出菇实验和分子标记等4个方面对两对配对单核和所获得的双核菌株进行分析和鉴定。结果表明:(1)通过双向核迁移的方法可以获得具有两个相同细胞核和不同受体细胞质的香菇双核菌株;(2)选择不同的受体细胞会对双核菌丝的菌落形态和出菇期产生明显的影响,表明香菇的这些性状可能会与细胞质中的某些因子有关。
Monokaryon mating tests between different strains of Lentinula edodes were carried out,and two pair dikaryotic strains were obtained by utilizing the mechanism of ＇bidirectional haploid nuclei migration＇.The strains were purified by isolation of single hyphal tips of dikaryotic strains and making use of ＇Buller phenomenon＇.Two pairs of monokaryotic and dikaryotic strains were analyzed by observations of colony shapes,microscopic examination,fruiting tests,and molecular markers.The results showed that the strains of L.edodes consisting of the same nuclei but different cytoplasm could be obtained,different strains with accepted cytoplasm of dikaryotic mycelia appeared to have different colony shapes and fruiting-date.The data indicated that the factors in cytoplasm were probably responsible for these phenomena.
出处
《菌物学报》
CAS
CSCD
北大核心
2011年第5期774-781,共8页
Mycosystema
基金
福建省科技重大专项(No.2008SZ0002)
公益类科研院所专项项目(No.2009R10009-1)
农业科技重点项目(No.2009N0028)
关键词
单-单杂交
双-单杂交
布勒现象
monokaryon mating
dikaryon-monokaryon mating
Buller phenomenon
