地塞米松和1,25-(OH)_2D_3对EC1细胞增殖和细胞周期的影响

Effects of dexamethasone and 1,25-(OH)_2D_3 on cellular proliferation and cycles of EC1 cells

目的:观察地塞米松(DEX)和1,25-(OH)2D3及2药联用对食管鳞状细胞癌EC1细胞增殖及周期的影响。方法:分别用10-7mol/LDEX与10-7mol/L1,25-(OH)2D3以及同种浓度2药联合处理EC1细胞48、72和96h,并设溶剂对照组。采用MTT法检测细胞增殖情况及流式细胞仪检测细胞周期变化。结果:1,25-(OH)2D3单独应用具有抑制EC1细胞增殖的作用(F1,25-(OH)2D3=51.185,P<0.001);DEX单独应用具有阻滞细胞周期的作用(FDEX=8.170,P=0.009)。DEX、1,25-(OH)2D3及时间3种因素两两间均存在交互作用(细胞增殖抑制:F1,25-(OH)2D3×DEX=11.017,P=0.003;F1,25-(OH)2D3×时间=3.668,P=0.041;FDEX×时间=7.887,P=0.002。G0/G1期细胞比率:F1,25-(OH)2D3×DEX=12.381,P=0.002;F1,25-(OH)2D3×时间=6.583,P=0.005;FDEX×时间=5.096,P=0.014),且两药间提示存在拮抗作用。未发现3因素之间存在交互作用(细胞增殖抑制:F1,25-(OH)2D3×DEX×时间=0.512,P=0.606。G0/G1期细胞比率:F1,25-(OH)2D3×DEX×时间=0.410,P=0.668)。结论:DEX和1,25-(OH)2D3单独及联合使用能对EC1细胞增殖起抑制作用,且使EC1细胞周期阻滞在G0/G1期。

Aim：To explore the impact of DEX,1,25-（OH）2D3 and DEX in combination with 1,25-（OH）2D3 on the cellular proliferation and cycles of EC1 cells in different periods of time.Methods：The EC1 cells were treated by 10-7 mol/L DEX,10-7 mol/L 1,25-（OH）2D3 and the combinations in same concentration for 48,72,and 96 h.The EC1 cells were treated by absolute ethyl alcohol in same level as the solvent control group.The morphological change of the cells was observed by optical inverted microscopy,the state of cellular proliferation was tested by MTT method,and the distribution of cell cycle was measured by flow cytometry.Results：The proliferation or cycle of EC1 cell were inhibited significantly in 1,25-（OH）2D3 group or DEX group （inhibition rate of cell proliferation：F1,25-（OH）2D3=51.185,P0.001;rate of cell staying in G0/G1 phase：FDEX=8.170,P=0.009）,and for DEX factor,1,25-（OH）2D3 factor ,time factor,there is interaction between any two factors（（inhibition rate of cell proliferation：F1,25- （OH）2D3×DEX=11.017,P=0.003;F1,25- （OH）2D3×time=3.668,P=0.041;FDEX×time =7.887,P=0.002.rate of cell staying in G0/G1 phase：F1,25- （OH）2D3×DEX=12.381,P=0.002;F1,25- （OH）2D3×time=6.583,P=0.005;FDEX×time=5.096,P=0.014）,but there is not interaction among time and the combination group（inhibition rate of cell proliferation：F1,25- （OH）2D3×DEX×time=0.512,P=0.606;rate of cell staying in G0/G1 phase：F1,25- （OH）2D3×DEX×time=0.410,P=0.668）.Conclusion：1,25-（OH）2D3,DEX and their combination could inhibit the cell proliferation and induce cell cycle to arrest in G0/G1 phase.