两头毛水提物对衰老小鼠心、肝及肾组织中MDA、GSH和SOD含量的影响

Effects of water extract from incarvillea arguta royle on levels of MDA,GSH and SOD in heart,liver and kidney of mice

目的:探讨两头毛水提物(IAR-Water)的抗氧化活性及抗氧化机制。方法:72只小鼠分成6组,每组12只,分别为空白组,模型组,阳性药物维生素E组(200mg/kg),IAR-Water低、中和高剂量(分别相当于药材1.9、3.8和7.6g/kg)组,给药同时,模型组、阳性药物维生素E组、IAR-Water低、中和高剂量组小鼠每天颈背部皮下注射D-半乳糖1g/(kg·d)建立衰老小鼠模型,空白组皮下注射等体积注射用水。7周后对小鼠心、肝和肾组织中丙二醛(MDA)、超氧化物歧化酶(SOD)和还原型谷胱甘肽(GSH)的含量进行测定。结果:各组小鼠心、肝、肾中MDA、SOD和GSH的含量比较,差异有统计学意义(P<0.05)。与模型组比较,阳性药物维生素E组和IAR-Water中和高剂量组心、肝、肾组织中MDA的含量下降,SOD和GSH的含量升高(P<0.05)。结论:IAR-Water可能通过清除羟自由基而发挥抗氧化作用。

Aim： To study the antioxidative activity and its mechanism of the water extract from incarvillea arguta royle（IAR）.Methods：A total of 72 mice were randomly divided into six groups（12 mice in each group）：the normal group,model group,positive medicine vitamin E group （200 mg/kg）,IAR-Water low,medium,high dose group （equivalent drugs 1.9,3.8,7.6 g/kg）.At the same time of administration,the model group,positive control group and reagent mice were injected D-galactose 1g/（kg·d） by SC,the normal group was injected the same volume of water.Seven weeks after administration,the levels of malondialdehyde（MDA）,superoxide dismutase（SOD）,and glutathione peroxidase（GSH） in tissues of heart,liver,and kidney were detected.Results：The levels of MDA,SOD,and GSH of 6 groups in tissues of heart,liver,and kidney were obviously different （P〈0.05）.The levels of MDA were lower and the levels of SOD and GSH were higher in positive medicine vitamin E group,IAR-Water medium,high dose groups than those in model group（P〈0.05）.Conclusion：IAR water extract has the antioxidative activity by eliminating oxygen free radicals.