大鼠脊髓体感诱发电位(SSEP)、皮层运动诱发电位(CMEP)的测定

Determination of Spinal Somatosensory Evoked Potential(SSEP) Cortical Motor Evoked Potential(CMEP)in Rats

目的：测定正常大鼠脊髓诱发电位及运动诱发电位,建立本研究大鼠诱发电位正常值数据库。方法：取40只SD大鼠,麻醉下分别行SSEP及CMEP检测。SSEP检测：电刺激一侧胫神经,在T12-13棘间韧带处记录感觉诱发电位。CMEP检测：刺激电极置于大鼠相应的人体头部C3、C4,记录电极置于大鼠后腿大鱼际处。结果：SSEP由一个正向波（P）和一个负向波（N）组成,潜伏期为P：（3.17±0.43）ms和N：（6.58±0.77）ms;波幅为P：（2.15±0.43）μV和N：（1.42±0.82）μV,P-N峰峰波幅（3.57±1.70）μV。CMEP由P1、N1、P2、N2组成,P1N1较为清楚,潜伏期为P1：（8.60±0.87）ms和N1：（9.81±1.02）ms;波幅为P1：（229.85±247.12）μV和N1：（143.15±130.41）μV,P1-N1峰峰波幅（373.00±342.53）μV。结论：大鼠SSEP及CMEP测定正常值潜伏期较稳定,可以作为病理实验对照参考,而波幅差异较大。

Objective：Determining the spinal somatosensory evoked potentials and cortical motor evoked potentials in normal rats to establish the unit normal database.Methods：40 SD rats under anesthesia were performed SSEP and CMEP testing.SSEP test：Sensory evoked potentials were recorded in T12~13 interspinous ligament while stimulating one side of the tibial nerve.CMEP test：stimulating electrodes were placed in the corresponding human head C3,C4,recording electrodes were placed at the thenar of rat hind legs.Results：SSEP consists of a positive wave（P）and a negative wave（N）.Wave latency：P（3.17±0.43）ms,N（6.58±0.77）ms;Amplitude：P（2.15±0.43）μV,N（1.42±0.82）μV,PN（3.57±1.70）μV.CMEP consists of P1,N1,P2,N2.P1N1 was more clearly.Wave latency：P1（8.60±0.87）ms,N1（9.81±1.02）ms.Amplitude：P1（229.85±247.12）μV,N1（143.15±130.41）μV,P1-N1（373.00±342.53）μV.Conclusion：The latency of normal rats SSEP and CMEP is stable,which can be used as the control reference,while the volatility is quite different.