佛手采后致病青霉的分离鉴定

Identification of Penicillium species causing post-harvest diseases of finger citron

佛手（Citrusmedica var.sarcodactylis Swingle）又名九爪木、五指橘、佛手柑,为芸香科柑橘属常绿小乔木。主产于闽、粤、川、浙等省,其中浙江金华＂金佛手＂最为著名,被称为＂果中之仙品,世上之奇卉＂。其果形美观、色泽金黄、香味浓郁,具有药用价值高、贮藏期长的特点[1],

Golden Finger citron（Citrusmedica var.sarcodactylis Swingle）,one of the traditional specialities of Jinhua,Zhejiang Province,has high ornamental and medicinal value.Green mould is the major fungus di-sease of Citrus fruits,causing substantial economic losses during the storage of post-harvest.To identify the exact species of the pathogenic fungus,an isolate belonged to genus Penicillium was isolated from the infected Golden Finger citron.Koch＇s postulation was completed by wounded-inoculation with the conidial suspension of Penicillium sp.on health Golden Finger.It was identified as Penicillium digitatum based on morphological characters and internal transcribed spacer（ITS） analysis.This result would be helpful for controlling post-harvest disease.