饲粮结构对山羊消化系统尿素转运蛋白-B表达的影响

Effects of Dietary Structure on Urea Transporter-B Expression in Digestive System of Goats

本试验旨在研究饲粮结构对山羊消化系统尿素转运蛋白-B(UT-B)表达的影响。选用18只装有永久性瘤胃瘘管的内蒙古白绒山羊半同胞羯羊,随机分为6组(每组3个重复,每个重复1只羊),45d后每组随机屠宰1只山羊,采用蛋白质印迹分析所采集的样品中UT-B的表达情况,并对34ku UT-B进行相对定量分析。结果表明:所有样品表达的UT-B处于30～55ku且均含有34ku的UT-B;饲粮高蛋白质水平可提高前胃(瘤胃、网胃和瓣胃)、回肠、盲肠盲囊和肾脏中34ku UT-B的表达;玉米膨化加工减少了34ku UT-B在回肠和肝脏的表达;各组织中34ku UT-B分布由高到低依次为肝脏、皱胃、网胃、瘤胃背囊、瘤胃腹囊、腮腺、瓣胃、十二直肠、盲肠、盲肠盲囊、回肠和肾脏。由此可知,通过改变饲粮结构可以调控山羊消化系统各组织中UT-B的表达,为进一步研究尿素循环和机体氮代谢提供依据。

This study was designed to investigate the effects of dietary structure on urea transporter-B（UT-B） expression in digestive system of goats.A total of 18 Inner Mongolia white cashmere goats with the same father were divided into 6 groups with 3 replicates per group and 1 goat per replicate.After 45 days,one goat was selected and euthanized in each group in a completely randomized design,and samples were taken for Western Blot for UT-B and for evaluation of the relative quantity of the 34 ku UT-B.The results showed as follows： the 30 to 55 ku UT-B were shown in all samples and all of them contained 34 ku UT-B;the expression of 34 ku UT-B in forestomach（rumen,reticulum and omasum）,ileum,blind sac of cecum and kidney were improved by high dietary protein level,but extrusion corn lowered the UT-B expression in the ileum and liver.Among organizations,the order of the intension for 34 ku UT-B is liver,abomasum,reticulum,ventral rumen,dorsal rumen,parotid salivary gland,omasum,duodenum,cecum,blind sac of cecum,ileum and kidney.Therefore,the expression of UT-B in the digestive system of goats can be adjusted by the variation of dietary structure,and it can provide a far-reaching foundation of research in urea cycle and nitrogen metabolism.