增施钙素对盐胁迫下西瓜幼苗生长和可溶性蛋白含量及组分的影响

Effect of supplementary calcium on the growth and the content and components of soluble protein of watermelon seedlings under salt-stress

采用水培方式,以盐敏感型的西瓜(Citrullus lanatus Mansfeld)品种‘秀丽’为试材,研究了营养液增补Ca2+对盐胁迫下西瓜幼苗生物量、可溶性蛋白含量和组分的影响。结果表明:50 mmol.L-1 NaCl胁迫下,营养液中Ca2+浓度由4 mmol.L-1增补到6和8 mmol.L-1时可明显提高盐胁迫幼苗植株的生物量,其分别达到对照水平的78.17%和89.50%,根系可溶性蛋白含量分别提高到对照水平的89.1%和104.3%,叶片可溶性蛋白含量分别提高到对照水平的92.4%和103.7%。同时,叶片28.5×103蛋白组分升高,107.4×103蛋白组分降低,根系的多种蛋白组分升高接近对照水平。因此,Ca2+对这些蛋白组分的表达影响作用可能是其缓解西瓜植株盐胁迫伤害的原因之一。

Salinity-sensitive mini-watermelon（Citrullus lanatus Mansfeld）cultivar＇Xiuli＇was used to study the effect of supplementary calcium on the growth and the content and components of soluble protein of watermelon seedlings grown under salt-stress by applying additional calcium to hydroponic nutrient solution.The results showed that the increasing Ca2＋ concentration from 4 mmol·L-1 to 6 and 8 mmol·L-1 could significantly increase biomass and soluble protein content of seedlings grown under 50 mmol·L-1 NaCl stress.Increasing Ca2＋ concentration increased the salt-stressed plant biomass to 78.2%,89.5% of control respectively,and soluble leaf protein content to 89.1%,104.3% of control,and soluble root protein content to 92.4%,103.7% of control.Moreover,increasing Ca2＋ concentration up-regulated 28.5×103 protein component and down-regulated 107.4×103 protein component in leaf,and up-regulated several root proteins components,nearly the same as the control.Therefore,the effects of supplementary calcium on these proteins components expression are possibly one of important reasons why calcium can alleviate salt-stress damage in watermelon seedlings.