与黄花马蹄莲互作过程中胡萝卜软腐果胶杆菌的差异表达蛋白分析

Proteomic analysis of Pectobacterium carotovorum subsp.carotovorum in the interaction with the host plant Zantedeschia elliotiana 'Black magic' in vitro

通过病原菌胡萝卜软腐果胶杆菌胡萝卜亚种与黄花马蹄莲组织的离体互作,运用双向电泳技术、质谱技术及Im-ageMaster 2D platinum 5.0(GE)软件对互作过程中蛋白质差异表达进行分析。结果表明:70个蛋白点在互作中存在表达差异,占蛋白点总数的18.13%。通过质谱分析发现:2个特异表达蛋白分子伴侣DnaK和1-脱氧木酮糖-5-磷酸合酶(DXS)均为代谢类蛋白;3个显著上调表达蛋白中ATP合酶α亚基和半胱氨酸合酶为代谢类蛋白,而β-内酰胺酶为抗生素类蛋白。

A proteomic approach was applied in this study on the changes of proteins expressed by the pathogen bacteria Pectobacterium carotovorum subsp.carotovorum（P.c.c）in the course of interation with the host plant tissue（Zantedeschia elliotiana＇Black magic＇）in vitro by using two-dimensional electrophoresis（2-DE）,mass technology and ImageMaster 2D platinum 5.0（GE）software.The results showed that,18.13% of the total protein spots（70/386）in the gels indicated expression high differentially.Totally 5 proteins were identified by mass spectrometry analysis;it was found that in addition to beta-lactamase（upregulating exprssed proteins）which was antibiotic protein;other four proteins all belonged to metabolic enzymes,among them,molecular chaperone DnaK and 1-deoxy-D-xylulose-5-phosphate synthase（DXS）were proteins specifically expressed;ATP synthase subunit alpha and cysteine synthase were upregulating exprssed proteins.