低聚异麦芽糖对产蛋鸡生产性能、盲肠微生物和免疫机能的影响

Effect of Dietary Isomalto-oligosaccharide on Performance,Caecal Microflora and Immune Function of Laying Hens

本试验旨在研究饲粮中添加低聚异麦芽糖(IMO)对产蛋鸡生产性能、盲肠微生物菌群、机体抗氧化和免疫机能的影响。试验选取600只体重和产蛋率相近的200日龄海兰褐产蛋鸡,随机分成5个处理(饲粮中分别添加0、0.1%、0.2%、0.4%和0.8%的IMO),每个处理6个重复,每个重复20只鸡。试验期8周。结果表明,与对照组相比,饲粮添加低剂量(0.1%和0.2%)IMO可在一定程度上改善平均蛋重、产蛋率、产蛋量和料蛋比,改善鸡蛋蛋白高度和哈夫单位;0.8%IMO组产蛋鸡的生产性能和鸡蛋品质均较低剂量组差。饲粮添加0.2%和0.4%IMO能显著增加产蛋鸡血浆球蛋白比例、降低尿素氮含量(P<0.05);0.2%IMO组能显著降低产蛋鸡盲肠食糜大肠杆菌的数量、增加乳酸杆菌和双歧杆菌的数量(P<0.05);0.2%和0.4%IMO组产蛋鸡血清中超氧化物歧化酶、谷胱甘肽过氧化物酶和总抗氧化酶活性显著升高(P<0.05);0.2%～0.8%IMO组产蛋鸡血浆免疫球蛋白A、免疫球蛋白M、免疫球蛋白G和溶菌酶均显著提高(P<0.05)。结果提示,饲粮添加IMO可优化产蛋鸡盲肠菌群结构,提高产蛋鸡抗氧化和免疫功能,改善生产性能。产蛋鸡饲粮中IMO的适宜添加量为0.2%。

The experiment was conducted to study the effect of dietary isomalto-oligosaccharide（IMO） on performance,caecal microflora,antioxidation and immune function of laying hens.Six hundred Hy-Line variety brown laying hens aged 200 days were randomly allotted into 5 groups,and each group was replicated 6 times,with 20 birds per replicate.Laying hens in the five groups were fed with the basal diet supplemented with 0,0.1%,0.2%,0.4% and 0.8% of IMO,respectively.The experiment lasted for 8 weeks.The results showed as follows： compared with the control group,average egg weight,laying rate,egg mass,feed/egg ratio,albumen height and Haugh unit of laying hens in 0.1% and 0.2% IMO groups were improved to a certain extent.The laying performance and egg quality of laying hens in 0.8% IMO group were lower than those in 0.1% and 0.2% IMO groups.Globulin/albumin ratio（Glb/Alb） in plasma of laying hens in 0.2% and 0.4% IMO groups was increased（P0.05）,while urea nitrogen content was reduced（P0.05）.The number of Lactobacillus and Bifidobacterium in caecum of laying hens in 0.2% IMO group was increased（P0.05）,while that of E.coli was decreased（P0.05）.Compared with the control group,the activities of superoxidedismutase（SOD）,glutathione peroxidase（GSH-Px）,and total antioxidative capacity（T-AOC） in serum of laying hens were significantly increased by 0.2% and 0.4% IMO supplementation in diets（P0.05）.When 0.2% to 0.8% IMO was added in diets,the contents of IgA,IgM and IgG and lysozyme activity in plasma of laying hens were all increased（P0.05）.In conclusion,dietary IMO can improve the performance,optimize caecal microflora,and enhance the anti-oxidative ability and immunological function of laying hens.The appropriate supplemental level of IMO in the diet of laying hens was 0.2%.