细胞毒性T淋巴细胞相关抗原4编码区49位点的基因多态性与宫颈癌遗传易感性的关系

Association between CTLA-4 gene polymorphism and susceptibility to cervical cancer

目的探讨细胞毒性T淋巴细胞相关抗原4（CTLA-4）编码区49位点的基因多态性与宫颈癌遗传易感性的关系。方法收集314例宫颈癌患者和320例正常对照者的外周静脉血标本，应用聚合酶链反应-限制性片段长度多态性法，检测CTLA-4＋49A／G多态位点的基因型。采用多因素Logistic回归法，分析基因多态性与宫颈癌发病风险的相关性以及与宫颈癌临床病理特征的关系。结果病例组中CTLA-4＋49A等位基因和AA基因型的频率分别为32．5％和9．6％，对照组中分别为25．8％和5．6％。携带CTLA-4＋49AA基因型者患宫颈癌的风险是GG基因型的2．06倍（95％CI为1．10—3．85，P=0．024）。CTLA-4＋49基因多态与宫颈癌临床分期、病理类型、分化程度、肿瘤大小和血清鳞状上皮细胞癌抗原水平等临床病理因素均无关（均P〉0．05）。结论CTLA-4基因有可能是宫颈癌的遗传易感基因。

Objective To investigate the association between CTLA-d ＋ 49A/G polymorphisms and the risk of susceptibility to cervical cancer. Methods A hospital-based case-control study was conducted. 314 cases with primary cervical cancer and 320 healthy controls were collected and genotyped by PCR-based RFLP for ＋49A/G polymorphisms in the CTLA-4 gene. Results The A allele and AA genotype of CTLA- 4 gene were 32.5% and 9.6% in the patients, and 25.8% and 5.6% in the controls, respectively. Subjects with CTLA-d ＋ 49AA genotype conferred a higher risk of cervical cancer （ OR = 2.06,95% CI： 1.10-3.85; P = 0. 024）. However, the correlation between AA genotype in CTLA-d polymorphisms and clinicopathological characteristics was not significant. Conclusion The results of this study suggest that CTLA4 gene is associated with cervical cancer risk and may be a susceptible gene of cervical cancer.