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Janus激酶1/胞外信号调节激酶通路参与C反应蛋白诱导的心肌细胞MMP-10活性上调 被引量：1

The Janus Kinase 1/Extracellular Signal-regulated Kinase Pathway IsInvolved in the Up-regulation of Matrix Metalloproteinase-10 Activityby C-reactive Protein in Cardiomyocytes
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摘要 为探讨心肌细胞中Janus激酶1(JAK1)在C反应蛋白(CRP)诱导的基质金属蛋白酶(MMP)-10活性上调过程中的作用,本研究以炎症细胞因子CRP诱导MMP-10上调的原代乳鼠心肌细胞为研究对象,使用JAK1的特异磷酸化抗体,用Western印迹技术检查JAK1的磷酸化水平的激活情况.应用Western印迹技术和酶谱法分别检测胞外信号调节激酶(ERK)的磷酸化水平和MMP-10的活性变化.实验分为4组:对照组,CRP组,CRP+抑制剂组和抑制剂组.结果显示,磷酸化的JAK1在CRP刺激后1/12 h即可出现,在1 h达到高峰,而总的JAK1不改变;JAK1抑制剂piceatannol可以使磷酸化的ERK水平减弱,也可以使MMP-10的活性明显降低(P<0.01).研究结果提示,JAK1是通过激活ERK,从而上调MMP-10的活性,为心力衰竭提供了一个可能的治疗靶点.
作者 崔传珏 魏英杰 史强 刘晓艳 白媛媛
机构地区 中国医学科学院北京协和医学院
出处 《中国生物化学与分子生物学报》 CAS CSCD 北大核心 2011年第9期885-888,共4页 Chinese Journal of Biochemistry and Molecular Biology
基金 国家自然科学基金项目(No.81041032) 国家重点基础研究发展规划(973计划,No.2010CB529506)~~
关键词 Janus激酶1 胞外信号调节激酶 基质金属蛋白酶-10 心肌细胞 C反应蛋白
分类号 R34 [医药卫生—基础医学]
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参考文献15

  • 1Chang F, Steehllan LS, Lee JT, et al. Signal transduction mediated by the Ras/Raf/MEK/ERK pathway from cytokine receptors to transcription factors: potential targeting for therapeutic intervention [ J]. Leukemia, 2003, 17 (7) : 1263- 1293.
  • 2Saxena NK, Sharma D, Ding X, et al. Concomitant activation of the JAK/STAT, PI3K/AKT, and ERK signaling is involved in leptin-mediated promotion of invasion and migration of hepatocellular carcinoma cells [ J]. Cancer Res, 2007, 67 (6) : 2497-2507.
  • 3Trimarchi T, Pachuau J, Shepherd A, et al. CNTF-evoked activation of JAK and ERK mediates the functional expression of T-type Ca^2+ channels in chicken nodose neurons [ J ]. J Neuroehem, 2009, 108 ( 1 ) : 246-259.
  • 4陈红芳,张亚梅,邓华瑜.乳腺癌细胞MDA-MB-231侵袭转移中JAK抑制对ERK的作用及意义[J].第二军医大学学报,2008,29(8):900-903. 被引量：5
  • 5崔传珏,魏英杰,张秀芳,史强,胡盛寿.乳鼠心肌细胞中C反应蛋白对基质金属蛋白酶-10表达调控的研究[J].中国循环杂志,2010,25(6):476-479. 被引量：6
  • 6Ghoreschi K, Laurence A, O'Shea JJ. Janus kinases in immune cell signaling [J]. Immunol Rev, 2009, 228(1 ) :273-287.
  • 7Pellegrini S, Dusanter FI. The structure, regulation and function of the Janus kinase (JAKs) and the signal transducers and activators of transcriptions (STATs) [ J]. Eur J Biochem, 1997, 248 : 615-633.
  • 8聂磊,韩梅,温进坤.JAK-STAT参与血管平滑肌细胞血管紧张素原和心钠素基因的转录激活[J].中国生物化学与分子生物学报,2006,22(1):70-76. 被引量：4
  • 9Neilson LM, Zhu J, Xie J, et al. Coactivation of Janus tyrosine kinase (Jak)1 positively modulates prolactin-Jak2 signaling in breast cancer: recruitment of ERK and signal transducer and activator of transcription (Stat)3 and enhancement of Akt and Stat5a/b pathways [ J]. Mol Endocrinol, 2007, 21 (9) : 2218- 2232.
  • 10Kim S, Choi JH, Lira HI, et al. EGF-induced MMP-9 expression is mediated by the JAK3/ERK pathway, but not by the JAK3/STAT-3 pathway in a SKBR3 breast cancer cell line [J]. Cell Signal, 2009, 21(6) : 892-898.

二级参考文献31

  • 1郑斌,温进坤,韩梅,周爱儒.hhlim促进DMSO诱导的P19细胞向心肌分化[J].生物化学与生物物理进展,2004,31(6):506-511. 被引量：4
  • 2聂磊,韩梅,温进坤.血管舒-缩肽在血管平滑肌细胞中的表达与调控[J].中国生物化学与分子生物学报,2005,21(1):94-100. 被引量：11
  • 3Fedak PWM,Verman S,Weasel RD,et al.Cardiac remodeling andfailure:From molecules to man.Cardiovasc Pathol,2005,14 (2):49-60.
  • 4Wei YJ,Cui Cj,Lainscak M,et al.Type-specific dysregulation of matrix metalloproteinases and their tissue inhibitors in end-stage,heart failure patients:relationship between MMP-10 and LV remodelling.J Cell Mol,2010,doi:10.1111/J.1582-4934.2010.01049.x.
  • 5Campian ME,Hardziyenka M,de Bruin K,et al.Early inflammatory response during the development of right ventricular heart failure in a rat model.Eur J Heart Fail,2010,12(7):653-658.
  • 6Kohno T,Anzai T,Naito K,et al.Impact of serum C reactive protein elevation on the left ventricular spherical change and the development of mitral regurgitation after anterior acute myocardial infarction.Cardiology,2007,107:386-394.
  • 7Montero I,Orbe J,Varo N,et al.C-reactive protein induces matrix metalloproteinase-1 and -10 in human endothelial cells:implications for clinical and subclinical atherosclerosis.J Am Coll Cardiology,2006,47:1369-1378.
  • 8Orbe J,Rodriguez JA,Calvayrac O,et al.Matrix Metalloproteinase10 upregulated by thrombin in endothelial cells and increased in patients with enhanced thrombin generation.Arterioscler Thromb Vase Biol,2009,29:2109-2116.
  • 9Clark IM,Swingler TE,Sampieri CL,et al.The regulation of matrix metalloproteinases and their inhibitors.Int J Biochem Cell Biol,2008,40:1362-1378.
  • 10Rodriguez JA,Orbe J,Lizarrondo SM,et al.Metalloproteinases and atherothrombosis:MMP-10 mediates vascular remodeling promoted by inflammatory stimuli.Front Biosci,2008,13:2916-2921.

共引文献12

同被引文献19

  • 1Kandel E S, Hay N. The regulation and activities of the multifunctional serine/threonine kinase Akt/PKB [J].Exp Cell Res, 1999, 253 (1):210-229.
  • 2Chen W S, Xu P Z, Gottlob K, et al. Growth retardation and increased apoptosis in mice with homozygous disruption of the Akt1 gene[J].Genes Dev,2001,15 (17):2203–2208.
  • 3Cho H, Thorvaldsen J L, Chu Q, et al. Akt1/PKBalpha is required for normal growth but dispensable for maintenance of glucose homeostasis in mice[J].J Biol Chem,2001, 276 (42):38349–38352.
  • 4Tschopp O, Yang Z Z, Brodbeck D, et al. Essential role of protein kinase B gamma (PKB gamma/Akt3) in postnatal brain development but not in glucose homeostasis[J].Development,2005,132 (13):2943–2954.
  • 5Easton R M, Cho H, Roovers K, et al. Role for Akt3/protein kinase B gamma in attainment of normal brain size[J].Mol Cell Biol,2005, 25 (5):1869–1878.
  • 6Dummler B, Hemmings B A. Physiological roles of PKB/Akt isoforms in development and disease [J].Biochem Soc Trans, 2007,35(Pt 2):231–235.
  • 7Manning B D, Cantley L C.AKT/PKB signaling: navigating downstream[J].Cell, 2007,129 (7):1261-1274.
  • 8Woodgett J R. Recent advances in the protein kinase B signaling pathway[J].Curr Opin Cell Biol,2005,17 (2):150-157.
  • 9Cho H, Mu J, Kim J K, et al. Insulin resistance and a diabetes mellitus-like syndrome in mice lacking the protein kinase Akt2 (PKB beta) [J].Science, 2001,192 (5522):1728-1731.
  • 10Gonzalez E, McGraw T E. Insulin-modulated Akt subcellular localization determines Akt isoform-specific signaling [J].Proc Natl Acad Sci U S A, 2009,106 (17):7004-7009.

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中国生物化学与分子生物学报
2011年 第9期

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