黄瓜无毛基因gl-2的遗传分析和定位

Genetic Analysis and Mapping of gl-2 Gene in Cucumber(Cucumis sativus L.)

以黄瓜(Cucumis sativus L.)有毛类型‘9110Gt’(P1)和无毛突变体‘NCG-042’(P2)为试材,对无毛基因gl-2进行遗传分析和基因定位研究。结果表明,黄瓜的有毛、无毛性状由一对核基因控制,有毛对无毛为显性。结合分离群体分组混合分析法(bulked segregant analysis,BSA),以F2为作图群体,筛选得到18对与黄瓜无毛基因gl-2相关的SSR引物,构建了gl-2基因的SSR连锁群,并将该基因定位在黄瓜第2染色体上,两侧最近的连锁标记为SSR10522和SSR13275,遗传距离分别为0.6cM和3.8cM。经过回交群体验证,SSR10522和SSR13275的正确率分别为94.4%和91.6%。

Genetic analysis and gene mapping were carried out on gl-2 gene in cucumber（Cucumis sativus L.）using 9110G（twith trichomes）and NCG-042（glabrous mutant）as experimental materials.The genetic analysis showed having trichomes or not is determined by a single nuclear gene,and the trait of having trichomes（Gl-2）is dominant to the glabrous（gl-2）in cucumber.Bulked segregant analysis（BSA） and simple sequence repeat（SSR）techonology were employed to mapping gl-2 gene of cucmber in F2 population.gl-2 gene was mapped to a linkage group with 11 SSR makers,corresponding to chromosome 2 of cucumber.The flanking markers SSR10522 and SSR132751 were linked to the gl-2 gene with genetic distances of 0.6 and 3.8 cM,respectively.The veracity of SSR10522 and SSR132751 was tested using BC1P2 population,and the accuracy rate for the two markers was 94.4% and 91.6%.