小苍兰ACC合成酶基因FhACS1在花中的时空表达特征

Organ-specific and Time-course Expression of ACC Synthase Gene FhACS1 in Freesia Flowers

研究了小苍兰‘上农金皇后’ACC合成酶基因FhACS1在花中的时空表达特征。结果表明,FhACS1基因在花朵不同器官、花朵的不同发育阶段以及瓶插期间在花朵不同器官中的表达量均存在明显的差异。FhACS1基因在花朵不同器官的表达量由高到低为:雄蕊>花瓣>雌蕊。不同发育阶段中FhACS1基因的表达特征表现为:花朵完全开放(0级)时表达量最高,大部分开放(1级)时次之,完全衰老花朵(-2级)中的表达量最低。将小苍兰鲜切花用蒸馏水瓶插,取花序上自基部起第3朵和第6朵小花为材料,研究了FhACS1基因随瓶插时间的表达谱,结果表明FhACS1基因在第6朵小花中的整体表达量显著高于第3朵。第3朵小花中FhACS1基因在花瓣、雄蕊、雌蕊中的表达均表现为先上升后下降,但最大表达量的出现时间存在差异;FhACS1基因在整朵花中的表达量变化与在花瓣中一致。第6朵小花花瓣中FhACS1基因的表达量持续上升,在雄蕊、雌蕊中的表达量则先上升后下降,达到最大表达量的时间分别为第3天和第6天;在整朵花中的表达变化也与在花瓣中的变化趋势一致。

Freesia hybrida＇Shangnong Jinhuanghou＇was used to investigate the organ-specific and time-course expression of 1-aminocyclopropane-1-carboxylate synthase gene FhACS1 in Freesia flowers.The results showed that significant differences presented in FhACS1 gene expression in different floral organs,florets at different developmental stage and florets during vase time.The organ-specific expression pattern of FhACS1 gene showed that the highest and the lowest expression were observed in stamens and pistil,respectively.The expression pattern of FhACS1 gene in florets at different developmental stages suggested that the highest and the lowest expression was observed in floret fully open（stage 0）and in florets at stage 4,respectively.To study the time-course expression pattern of FhACS1 gene in Freesiaflorets,the 3rd and 6th bud on inflorescence were used.The results suggested the expression in the 6th bud was significantly higher than that in the 3rd bud.For the 3rd bud,the expressions in petals,stamens and pistil were first increased and then decreased,but the highest expression in each floral organ occurred at different time;expression pattern in whole bud was consistent with that in petal.For the 6th bud,expressions of FhACS1 gene continued to rise in petals,and first increased and then decreased in stamens and pistil;the highest expressions in stamens and pistil occurred at the 3th and 6th day,respectively;the expression pattern in whole bud was similar with that in petals.